高脂饮食诱导的C57BL/6J幼鼠肾足细胞中Synaptopodin的表达及意义  

Expression of Synaptopodin in podocytes of high-fat diet C57BL/6J young mice

作　　者：沈健[1] 臧东钰[2] 李晓明[1] 

机构地区：[1]辽宁医学院组织胚胎学教研室,辽宁锦州121001 [2]辽宁医学院附属第三医院胸外科,辽宁锦州121001

出　　处：《解放军医学院学报》2015年第9期940-943,共4页Academic Journal of Chinese PLA Medical School

基　　金：辽宁省高等学校优秀人才支持项目(LJQ2013092);辽宁医学院校长基金项目(XZJJ20130243)~~

摘　　要：目的探讨足细胞骨架蛋白Synaptopodin在高脂饮食所致肾发育期足细胞损伤中的表达及意义。方法 32只3周龄雄性C57BL/6J幼鼠随机分为正常饮食组和高脂饮食组,分别给予标准饲料与高脂饲料喂养4周。检测葡萄糖耐量水平、尿微量白蛋白与尿肌酐;观察小鼠肾的病理形态学改变,应用免疫组织化学和免疫印迹技术检测小鼠肾足细胞中Synaptopodin的表达变化。结果与正常饮食组相比,高脂饮食组小鼠体质量显著增加,葡萄糖调节受损,尿微量白蛋白与尿肌酐比率增高。镜下可见肾小球肥大,肾小囊腔内渗出,系膜基质轻度增生等病理改变。同时足细胞Synaptopodin的表达明显降低。结论 Synaptopodin在高脂饮食诱导的C57BL/6J幼鼠肾足细胞中表达显著降低,并出现蛋白尿。Objective To investigate the expression and significance of Synaptopodin resulting in podocyte injury in high-fat diet C57BL/6J mice. Methods Thirty-two male C57BL/6J mice were randomly divided into two groups. The standard group was fed with basic diet and high-fat diet group was fed with high-fat diet for 4 weeks. Body weight and urine protein changes were measured. Insulin resistance was detected by glucose tolerance test. The kidney tissue morphological changes were examined by HE staining. The expression of Synaptopodin was examined by immunohistochemistry and western blotting. Results In high-fat diet group, the body weight was higher than the standard group, and there was significant decrease in glucose tolerance levels and rise in urinary albumin/ creatinine ratio. HE staining results showed that there were glomeruli hypertrophy, mesangial matrix hyperplasia, and glomerulosclerosis. Compared with standard group, the expression of Synaptopodin in high-fat diet group reduced significantly in podocyte. Conclusion The expression of Synaptopodin in podocyte of high-fat diet C57BL/6J mice reduces significantly, which may lead to proteinuria.

关 键 词：高脂饮食 足细胞 突触足蛋白 免疫组化 免疫印迹 

分 类 号：R587[医药卫生—内分泌]