天然虾青素对大鼠肠缺血再灌注损伤的保护作用  

Protective effect of Astaxanthin on intestinal ischemia reperfusion injury in rat

作　　者：喻苗[1] 金镭[1] 吕艳锋[1] 禹化龙[1] 王建新[1] 

机构地区：[1]山东大学第二医院肛肠外科,山东济南250033

出　　处：《中国现代普通外科进展》2015年第8期589-592,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨天然虾青素(ATX)对实验性大鼠肠缺血再灌注损伤的保护及作用机制。方法 :48只成年健康雄性SD大鼠随机分为假手术组(Sham组)、肠缺血再灌注组(IIR组)、高剂量ATX治疗组、低剂量ATX治疗组4组。Sham组仅分离肠系膜上动脉(SMA)而不夹闭;IIR组与ATX组于分离SMA后利用无损伤血管夹夹闭根部30 min、再灌注60 min诱导缺血再灌注损伤。两ATX治疗组分别于术前2 d给予ATX灌胃,剂量分别为100 mg/(kg·d)和50 mg(kg·d),连续灌胃2 d;Sham组及IIR组则均于同期给予相同剂量的生理盐水灌胃。4组大鼠于术后6 h处死,比较小肠组织丙二醛(MDA)、超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)水平及血清中肿瘤坏死因子(TNF)-α、IL-1β、IL-6水平变化。结果:与Sham组及IIR组比较,两ATX治疗组小肠组织MDA水平、MPO活性及血清TNF-α、IL-1β、IL-6明显下降(P<0.05),而SOD活性则有明显上升(P<0.05),且高剂量组作用较低剂量组明显。结论:ATX可以减轻肠缺血再灌注损伤而起保护作用,其机制可能与抗氧化及抑制炎症反应相关。Objective：To explore the protective effect of Astaxanthin （ATX） on intestinal ischemia reperfusion injury （ⅡR） in rat,and find possible relationship to oxidative stress and inflammatory factors.Methods：36 SD rats were divided to four groups,as Sham group,ⅡR group,and two ATX treatment group （100 mg/kg and 50 mg/kg）.The rats in two ATX group were dividely given 100mg/（kg· d） and 50mg/（kg· d） ATX solution（1 mL） by intragastric administration since 2 d before surgery,while other groups accept similar dose of saline.After 6 hours from operations,all rats were sacrificed,and liver MDA,SOD,MPO and serum TNF-α,IL-1 β,IL-6 were tested by ELISA kit.Results：The appliance of ATX gave rise to decreases in liver MDA,MPO and serum TNF-α,IL-1 β,IL-6,while SOD increased （P＜0.05）.The higher dose of ATX brought about more obvious effects.Conclusion：ATX can protect intestinie after ⅡR by anti-oxidation and inhibiting inflammation.

关 键 词：缺血再灌注 小肠 虾青素 大鼠 

分 类 号：R656.7[医药卫生—外科学] R657.2[医药卫生—临床医学]