灯盏花素联合骨髓间充质干细胞移植促进脑梗死大鼠神经功能恢复  

Breviscapine combined with bone marrow mesenchymal stem cell transplantation promotes neurological recovery of rats with cerebral infarction

作　　者：赵巍[1] 刘学政[1] 杨廷双[2] 

机构地区：[1]天津中医药大学第一附属医院,天津市300073 [2]天津医科大学总医院滨海医院放射科,天津市300052

出　　处：《中国组织工程研究》2015年第32期5134-5139,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:灯盏花素治疗脑梗死疗效确切、不良反应少、远期疗效稳定、毒副作用少,能够改善脑梗死后中枢神经系统受损区的微环境。目的:探讨灯盏花素注射液联合骨髓间充质干细胞移植对脑梗死神经功能恢复及生长相关蛋白43表达的影响。方法:将60只大脑中动脉闭塞模型SD大鼠随机分为脑梗死组、骨髓间充质干细胞移植组和联合组。建模6 h后通过尾静脉注射1 m L PBS、1 m L骨髓间充质干细胞悬液(2.5×106)、1 mL骨髓间充质干细胞悬液(2.5×106)+灯盏花素注射液75 mg/kg,连续注射5 d,1次/d。结果与结论:移植后2周,免疫荧光法观察到BrdU阳性标记的骨髓间充质干细胞主要集中于梗死灶周围且联合组的BrdU阳性细胞数量多于骨髓间充质干细胞组及脑梗死组(P<0.01);移植后1,2,3周联合组的神经功能障碍评分明显低于骨髓间充质干细胞组及脑梗死组(P<0.05);移植后2周,与骨髓间充质干细胞组及脑梗死组比较,联合组的脑梗死面积明显减小,水肿程度明显减轻,生长相关蛋白43的表达明显增高(P<0.05)。光镜下联合组脑梗死组织中胶质细胞明显增生,脑组织水肿有明显减轻。结果表明灯盏花素注射液联合骨髓间充质干细胞可明显减轻脑梗死面积及水肿程度,促进大鼠脑梗死后神经功能恢复及梗死灶生长相关蛋白43的表达。BACKGROUND：Breviscapine treatment of cerebral infarction has curative effects, few side effects, stable long-term efficacy and few side effects, which can improve the micro-environment of damaged central nervous system after cerebral infarction. OBJECTIVE：To investigate the effects of breviscapine injection combined with bone marrow mesenchymal stem cel transplantation on neurological recovery and growth-associated protein 43 expression in rats after cerebral infarction. METHODS：Sixty Sprague-Dawley rats undergoing middle cerebral artery occlusion were randomized into cerebral infarction group, cel transplantation group and combined group. At 6 hours after modeling, 1 mL PBS, 1 mL bone marrow mesenchymal stem cel suspension （2.5×106）, and 1 mL bone marrow mesenchymal stem cel suspension （2.5×106）＋75 mg/kg breviscapine injection were respectively injected via the tail vein in the three groups, once a day, continuously for 5 days. RESULTS AND CONCLUSION：At 2 weeks after transplantation, BrdU-positive bone marrow mesenchymal stem cels were mainly gathered in the peri-infarction region, and the number of positive cels was higher in the combined group than the other two groups （P 〈 0.01）. At 1, 2, 3 weeks after transplantation, the neurological deficit scores were significantly lower in the combined group than the other two groups （P 〈 0.05）. At 2 weeks＆nbsp;after transplantation, the combined group had smaler infarct size, milder edema, and higher expression of growth-associated protein 43 as compared with the other two groups （P 〈 0.05）. Under light microscope, glial cels proliferated dramaticaly and brain edema significantly reduced in the combined group. These findings indicate that breviscapine injection combined with bone marrow mesenchymal stem cel transplantation can significantly reduce infarct size and brain edema, promote neurological recovery and increase the expression of growth-associated protein 43 in rats with cerebral infarction.

关 键 词：干细胞 骨髓干细胞 灯盏花素注射液 骨髓间充质干细胞 生长相关蛋白43 脑梗死 大鼠 神经功能 

分 类 号：R394.2[医药卫生—医学遗传学]