卵巢癌细胞中CD90^+肿瘤干细胞的生物学特性  

Biological characteristics of CD90^+ tumor stem cells in ovarian cancer cells

作　　者：蒋小芒 赵娜 龙敏[2] 

机构地区：[1]西安市长安医院,陕西省西安市710061 [2]西安医学院附属第一医院,陕西省西安市710077

出　　处：《中国组织工程研究》2015年第32期5193-5198,共6页Chinese Journal of Tissue Engineering Research

基　　金：西安市卫生局科技攻关课题(J2013108)~~

摘　　要：背景:肿瘤干细胞与卵巢癌的发生和发展等之间存在十分密切的联系,CD90+是一种重要的肿瘤干细胞标志物。目的:探索卵巢癌细胞中CD90+肿瘤干细胞生物学特性。方法:获得原代卵巢癌细胞并进行分析,对人卵巢癌细胞系SKOV3和原代卵巢癌细胞的CD133和CD90阳性率进行流式细胞术检测,分选获得CD90+和CD90-细胞,并通过反转录PCR法对其干细胞和上皮间质化相关基因mR NA的相对表达情况进行检测。通过Transwell小室侵袭试验对细胞侵袭能力进程观察,利用克隆形成试验对细胞增殖分化能力进行观察,利用悬浮成球试验对干细胞潜能进行观察。并通过免疫缺陷小鼠体内有限稀释成瘤试验,对成瘤时间以及成瘤率进行观察。结果与结论:SKOV3细胞的CD133和CD90阳性率均显著低于原代卵巢癌细胞。SKOV3细胞系CD90+干细胞相关基因mR NA相对表达CD133和OCT4均显著高于SKOV3细胞系CD90-干细胞;原代卵巢癌细胞CD90+干细胞中CD44、CD133、乙醛脱氢酶1和OCT4均显著高于原代卵巢癌细胞CD90-干细胞。SKOV3细胞系CD90-和CD90+干细胞的上皮间质化相关基因mR NA相对表达水平差异有显著性意义,原代卵巢癌细胞CD90-和CD90+干细胞的上皮间质化相关基因mR NA相对表达水平差异有显著性意义。随着接种细胞数量的增加,CD90-和CD90+细胞成瘤率呈现出不断上升的情况,成瘤时间则不断下降。其中,CD90+干细胞的上升较之CD90-干细胞更为显著。SKOV3细胞系和原代卵巢癌细胞的CD90+干细胞穿膜细胞数、细胞克隆数、悬浮成球数量均显著大于CD90-干细胞。提示卵巢癌细胞中CD90+干细胞可高表达干细胞相关基因和间质属性基因,并具备较高的侵袭、增殖分化能力以及成瘤能力和较大的干细胞潜能。BACKGROUND：There is a close connection between the occurrence and development of tumor stem cels and ovarian cancer. CD90＋ is an important tumor stem cel marker. OBJECTIVE： To explore the biological characteristics of CD90＋ tumor stem cels in ovarian cancer cels. METHODS：The CD133 and CD90 positive rate of SKOV3 and primary ovarian cancer cels were detected by flow cytometry. The CD90＋ and CD90- relative expression in stem cels and epithelium was detected by RT-PCR. Transwel invasion assay was employed to observe the cel invasion ability, clone formation test was done to observe cel proliferation and differentiation capacity, suspension bal test was adopted to observe pluripotent stem cels. The tumor formation time and tumor formation rate were observed by limited tumor dilution in immunodeficient mice. RESULTS AND CONCLUSION：The positive rates of CD133 and CD90 in SKOV3 were significantly lower than those in primary ovarian cancer cels. The expression of CD133 and OCT4 in CD90＋cels of SKOVS was significantly higher than that in CD90-cels of SKOVS. The expression of CD44, CD133, acetaldehyde dehydrogenase-1 and OCT4 in CD90＋stem cels of primary ovarian cancer cels was significantly higher than that in CD90-stem cels of primary ovarian cancer cels. There were significant differences in the＆nbsp;epithelial-mesenchymal related gene expressions between CD90-and CD90＋stem cels of SKOV3 and primary ovarian cancer cels. With the increase of inoculated cels, the tumor formation rate of CD90-and CD90＋ cels was increased continuously, but the tumor formation time was decreased. The tumor rate of CD90-cels was lower than that of CD90＋cels. The number of transmembrane cels, cel clones and suspended cel bals was significantly higher in the CD90＋ stem cels than the CD90-stem cels. These findings indicate that in ovarian cancer cels, CD90＋stem cels can highly express stem cel-related genes and epithelial-mesenchymal related genes, which have a higher invasion, proliferation and differentiation a

关 键 词：干细胞 肿瘤干细胞 卵巢癌 肿瘤细胞 肿瘤干细胞标志物 生物学特性 上皮间质转化 CD90+细胞 

分 类 号：R394.2[医药卫生—医学遗传学]