基因工程菌K88-K99融合蛋白高密度发酵工艺的初步研究  

Preliminary Study on High Density Fermentation Thchnology of Gene Engineering Bacteria K88-K99 Fusion Protein

作　　者：谢金文[1,2] 李书光[1,2] 肖跃强[1,2] 王文秀[1,2] 唐娜[1,2] 董林[1,2] 李峰[1,2] 沈志强[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院/山东省畜禽用蜂胶疫苗研究开发推广中心,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600

出　　处：《动物医学进展》2015年第10期43-47,共5页Progress In Veterinary Medicine

基　　金：山东省自然科学基金青年基金项目(ZR2012CQ012;ZR2013CQ006;ZR2014CQ009;ZR2014CQ010);山东省现代农业产业技术体系生猪创新团队项目(SDAIT-06-011-14);滨州市2013年科学技术发展计划项目(2013GG0304)

摘　　要：通过培养基LB、改良LB、改良LB+微量盐、改良Minca和2×YT+10g/L葡萄糖5种培养基进行筛选,设定培养基初始pH分别为6.0、6.5、7.0和7.5四个梯度进行蛋白表达,用全自动微生物生长仪每隔30min测定菌液的OD值,最终确定2×YT+5g/L的葡萄糖生长菌数较多,细菌生长速度快,pH7.0时蛋白表达量较高,并且通过细菌生长曲线得出最佳诱导时机为细菌培养开始后3.0h^3.5h,诱导时长4.0h,溶氧量控制在30%,为ETEC大肠埃希菌高密度发酵工艺的研究提供了基础数据。The five kinds of media,LB,improved LB,improved LB＋ trace salt solution,improved Minca and 2＆#215;YT＋10 g/L glucose were screened,and the initial pH of medium were set as 6.0,6.5,7.0 and 7.5 for protein expression.With automatic microorganism growth instrument,OD values of bacterial liquid were measured every half an hour.Finally 2 ＆#215; YT ＋ 5 g/L glucose and pH7.0 were confirmed for the growth of bacterial protein expression.The optimal induction time was in 3.0 h-3.5 h after culture by bac-terial growth curve,induction time 4.0 h,oxygen control at 30%,for ETEC E.coli to provide basic data for the research of high density fermentation.

关 键 词：K88-99 培养基 高密度发酵 融合蛋白 

分 类 号：S852.612[农业科学—基础兽医学]