早产兔生发基质-脑室内出血模型的建立及神经行为学评价  

Establishment of GMH-IVH Preterm Rabbit Model and Neurobehavioral Evaluation

作　　者：暴丽莎[1] 刘芳[1] 

机构地区：[1]解放军白求恩国际和平医院新生儿科NICU,石家庄050082

出　　处：《临床误诊误治》2015年第9期89-91,共3页Clinical Misdiagnosis & Mistherapy

摘　　要：目的建立早产兔生发基质-脑室内出血(germinal matrix hemorrhage-intra-ventricular hemorrhage,GMH-IVH)的动物模型,并进行神经行为学评价。方法将妊娠29 d兔进行剖宫产,迅速取出幼兔,随机分为实验组及对照组,每组各18只。生后3 h实验组予50%丙三醇腹腔注射,对照组接受等剂量0.9%氯化钠注射液腹腔注射,均于出生后24 h接受头颅彩色多普勒超声检查。实验组发生GMH-IVH的早产兔为GMH-IVH模型组,对照组未发生GMH-IVH的早产兔为正常对照组,观察GMH-IVH的发生率并进行神经行为学评价。结果早产兔GMH-IVH的发生率实验组、对照组分别为83.33%(15/18)、11.11%(2/18),两组比较差异有统计学意义(P<0.01);早产兔出生后24 h在颅神经、运动、意识等神经行为学检测评分方面GMH-IVH模型组与正常对照组比较差异无统计学意义(P>0.05)。结论早产兔予腹腔注射丙三醇可建立GMH-IVH动物模型,此模型符合早产儿GMH-IVH的临床行为改变。Objective To establish the preterm rabbit of germinal matrix hemorrhage-intra-ventricular hemorrhage （ GMH-IVH） . Methods 29 premature rabbits were given birth by cesarean section. Then the premature rabbits were randomly divided into two groups;experimental group （n=18） and control group （n=18）. Three hours later, the premature rabbits in experimental group were given intra-peritoneal injection of 50% glycerol （10 ml/kg）, the preterm rabbits in control group were given intra-peritoneal injection with the same dose of saline. Both groups received the Cranial ultrasound scan check at 24 h after birth. Premature rabbits in experimental group undergoing GMH-IVH was called GMH-IVH model group, the control group not undergoing GMH-IVH in premature rabbits was called normal control group. Presence of GMH-IVH and neurobehavioral assess-ment were checked. Results In experimental group, the incidence rate of GMH-IVH was 83. 33%（15/18） and the incidence rate of GMH-IVH was 11. 11% （2/18） in control group. The incidence rate of GMH-IVH in experimental group was significant-ly higher than that of control group （P〈0. 01）. The Premature rabbits accepted neurobehavioral assessment on cranial nerves, consciousness and gait at 24 hours after birth. There was no significant difference in the neurobehavioral testing between the nor-mal control group and the GMH-IVH model group （P＆gt;0. 05）. Conclusion We have established animal models. The changes in this model are similar with clinical behavior characteristics of GMH-IVH in preterm children.

关 键 词：生发基质-脑室内出血 模型 动物 早产儿 神经行为学表现 超声检查 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]