烟酰胺腺嘌呤二核苷酸激酶2在恶性脑肿瘤中的普遍标志性表达  

Nicotinamide adenine dinucleotide kinase 2 is highly expressed in malignant brain tumors

作　　者：高维[1,2,3] 董士业 潘飞燕[1,2,3] 刘丹慧 

机构地区：[1]温州医科大学检验医学院 [2]生命科学学院 [3]浙江省医学遗传学重点实验室,浙江温州325035

出　　处：《解剖学报》2015年第5期587-590,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(81301744);温州医科大学校内课题资助项目(QTJ06011)

摘　　要：目的检测烟酰胺腺嘌呤二核苷酸激酶2(NADK2)在恶性脑肿瘤中的表达。方法使用免疫组织化学法检测了NADK2在75例恶性脑肿瘤中的表达。结果本实验检测了NADK2在75例恶性脑肿瘤中的表达情况,包括55例星形细胞瘤、13例少突神经细胞瘤、3例髓母细胞瘤和4例室管膜瘤,74例样本中NADK2的表达都明显高于正常对照组织。对免疫组织化学结果进行吸光度分析,比较NADK2在不同肿瘤类型和不同分级的脑肿瘤中的表达,结果表明,NADK2在恶性脑肿瘤中的表达与肿瘤类型和分级无关。结论高表达NADK2是恶性脑肿瘤的一个普遍特征。Objective To investigate the nicotinamide adenine dinucleotide kinase 2 （NADK2） expression level in malignant brain tumors. Methods Immunohistochemistry analysis was used to analyze the NADK2 expression level in 75 malignant brain tumors including 55 of astrocytoma, 13 of oligodendroglioma, 3 of medulloblastoma and 4 of ependymoma. The integrated absorbance （IA） was measured by ImageJ software. Results The immunohistochemistry results showed that 74/75 tumor samples showed strong NADK2 staining. The IA results suggested that there was no significant difference between different brain tumor types as well as grades. Conclusion The results indicate that high expression of NADK2 is a general characteristic of malignant brain tumors.

关 键 词：烟酰胺腺嘌呤二核苷酸激酶2 恶性脑肿瘤 免疫组织化学 人 

分 类 号：R739.41[医药卫生—肿瘤]