微小RNA-203在胶质母细胞瘤干细胞中的表达及其对细胞增殖的影响  

The expression of microRNA-203 in glioblastoma multiforme stem cells and its impact on cell proliferation

作　　者：邓一帆[1] 祝刚[1] 黄小山[1] 李百升[1] 秦忠宗[1] 

机构地区：[1]惠州市中心人民医院神经外科,广东516001

出　　处：《中华实验外科杂志》2015年第9期2085-2087,共3页Chinese Journal of Experimental Surgery

基　　金：广东省医学科研基金资助项目（B2014417）;惠州市科技计划资助项目（20140802）

摘　　要：目的 培养胶质母细胞瘤干细胞,检测微小RNA-203（miR-203）在胶质母细胞瘤干细胞中的表达,并观察其对肿瘤干细胞增殖的影响.方法 对胶质母细胞瘤组织行体外原代培养,以CD133为标志物,免疫磁珠法分选胶质母细胞瘤干细胞;免疫荧光染色检测分选所得细胞的CD133、巢蛋白（nestin）、神经胶质纤维酸性蛋白（GFAP）、微管相关蛋白2（MAP2）的表达,从而鉴定肿瘤干细胞.反转录-聚合酶链反应（RT-PCR）检测分选后CD133＋细胞、CD133-细胞miR-203的表达;将miR-203模拟体和无意义寡核苷酸链（NC）分别转染胶质母细胞瘤干细胞作为miR-203、NC组,细胞计数试剂盒（CCK-8）法检测转染后24、48、72、96、120 h细胞生存率;流式细胞仪细胞检测转染后3d细胞凋亡率.结果 培养的胶质母细胞瘤干细胞成球样生长,表达干细胞标志物CD133、Nestin,分化后细胞表达星形胶质细胞的标志物GFAP、神经元的标志物MAP2;分选后CD133＋细胞中miR-203的表达低于CD133-细胞;转染miR-203后24、48、72、96、120 h组的细胞生存率均降低,差异有统计学意义（P＜0.05）;流式细胞仪检测显示miR-203组的细胞凋亡率为（9.74±2.81）％,高于NC组的（3.95±0.91）％,差异有统计学意义（P＜0.05）.结论 上调miR-203可能成为针对胶质母细胞瘤干细胞的基因治疗策略.Objective To isolate glioblastoma multiforme stem cells （GBM-SCs） from glioblastoma multiforme （GBM） specimens and to investigate the expression of microRNA （miR）-203 in GBM stem cells and its impact on cell proliferation.Methods CD133 ＋ cells were separated using magnetic cell sorting technique （MACS） after primary culture.Reverse transcription-polymerase chain reaction （RT-PCR） was used to analyze miR-203 expression in CD133 ＋ cells.Lipofectamine RNAiMAX was used to transfect miR-203 mimic and scrambled control oligonucleotides into GBM-SCs.The proliferation ability of miR-203 treated GBM-SCs was determined by CCK-8 and the apoptosis ratio was detected by cell flow cytometry.Results GBM-SCs isolated from GBM specimens formed GBM spheres,expressed markers associated with neural stem cells,and possessed the capacity for self-renewal and multilineage differentiation.MiR-203 expression was down-regulated in CD133＋ cells relative to CD133-cells.MiR-203 can repress GBM-SCs growth and induce apoptosis.Conclusion Reactivation of miR-203 expression suggests novel therapeutic strategies for GBM-SCs.

关 键 词：胶质母细胞瘤 胶质母细胞瘤干细胞 微小RNA-203 细胞增殖 

分 类 号：R730.264[医药卫生—肿瘤]