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作 者:陈玲[1] 宋佳[2] 孙巧[2] 彭维恒[1] 黄徐英[1] 任立权[1] 陈继革[3]
机构地区:[1]武汉市普爱医院,430034 [2]武汉轻工大学生物与制药工程学院 [3]华中科技大学同济医学院附属同济医院创伤外科
出 处:《中华实验外科杂志》2015年第9期2092-2095,共4页Chinese Journal of Experimental Surgery
基 金:武汉市临床医学科研项目(WZ15Z08)
摘 要:目的 观察竹节参皂苷Ⅴ对损伤的PC-12细胞的修复作用以及对淀粉样前体蛋白样蛋白-1(APLP-1)和去整合素-金属蛋白酶-17(ADAM-17)基因表达的影响.方法 利用β-淀粉样蛋白25-35(Aβ25-35)诱导制备PC-12细胞损伤模型,噻唑蓝(MTT)法测定竹节参皂苷Ⅴ对受损PC-12细胞增殖活力的恢复作用,实时荧光定量聚合酶链反应(FQ-PCR)测定竹节参皂苷Ⅴ对PC-12细胞中APLP-1、ADAM-17基因表达的影响.结果 终质量浓度为15 μmol/L的Aβ25-35在作用于PC-12细胞12、24、48 h时,对细胞增殖的抑制率分别为13.15%、22.80%和9.80%,与空白组比较差异有统计学意义(P<0.05).在Aβ25-35诱导的细胞损伤模型(24h)的基础上,竹节参皂苷Ⅴ作用6、12、18、24h时PC-12细胞的增殖率与模型组比较分别增高5.53%、7.74% (P<0.05)、9.60%(P<0.01)和2.07%.模型组中PC-12细胞中APLP-1基因的相对表达量较空白组明显上调,ADAM-17基因的相对表达量则明显下调;竹节参皂苷Ⅴ给药组中APLP-1基因的相对表达量相对模型组明显下调,而ADAM-17基因明显上调.结论 竹节参皂苷Ⅴ对Aβ25-35诱导损伤的PC-12细胞有修复作用,推测是通过下调APLP-1的相对表达量,使淀粉样前体蛋白(APP)低量表达,并上调ADAM-17基因的相对表达量,使APP朝着非Aβ生成途径降解的方式来修复损伤的PC-12细胞.Objective To explore the effect of chikusetsusaponin Ⅴ on the repair of the injured PC-12 cell line and the expression of Amyloid precursor-like protein 1 (APLP-1) and A disintegrin and metalloproteinase domain 17 (ADAM-17).Methods PC-12 cell injury model was induce by Amyloid beta 25-35 (Aβ25-35).Methyl thiazol tetrazolium (MTT) was applied to analyze the repair effect of chikusetsusaponin Ⅴ on damaged proliferation of PC-12 cell line,while real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to assay the influence of chikusetsusaponin Ⅴ on the expression of APLP-1 and ADAM-17.Results Aβ25-35 (15 μmol/L) inhibited proliferation of PC-12 cells by 13.15%,22.80% and 9.80% respectively at 12,24,and 48 h after treatment,which had significant difference compared to control group.Chikusetsusaponin Ⅴ could protect PC-12 cell line against the cytotoxicity of Aβ25-35 through increasing cell proliferation by 5.53%,7.74% (P < 0.05),9.60% (P <0.01) and 2.07% at 6,12,18 and 24 h time points,respectively.The expression level of APLP-1 gene in model groups was obviously higher than that in control groups,while ADAM-17 gene was obviously lower than the control.Moreover,APLP-1 gene expression in PC12 cell line treated with chikusetsusaponin Ⅴ was strikingly down-regulated,while ADAM-17 gene was up-regulated.Conclusion It is indicated that chikusetsusaponin Ⅴ could repair the proliferation of PC-12 cell line injured by Aβ25-35 through down-regulating the APLP-1 gene and up-regulating ADAM-17 gene.
关 键 词:竹节参皂苷Ⅴ PC-12细胞 淀粉样前体蛋白样蛋白-1 去整合素-金属蛋白酶-17 基因表达
分 类 号:R742[医药卫生—神经病学与精神病学]
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