MDA-MB-468和SK-BR-3乳腺癌细胞建立裸鼠皮下种植瘤的生物学功能  

Biological functions of the nude mice subcutaneous tumor established by MDA-MB-468 and SK-BR-3 breast cancer cells

作　　者：赵期康[1] 吴颖[1] 莫雪丽 熊兵红[1] 

机构地区：[1]北京大学首钢医院乳腺科,100144

出　　处：《中华实验外科杂志》2015年第9期2146-2149,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 建立SK-BR-3和MDA-MB-468裸鼠皮下种植瘤模型,观察两种乳腺癌细胞株在建立裸鼠皮下移植瘤中的成瘤性和生物学与形态学特征.探讨阿霉素缓释剂对两种乳腺癌细胞裸鼠皮下种植瘤的生物学功能的影响.方法 将80只裸小鼠随机分为MDA-MB-468组和SK-BR-3组,MDA-MB-468组小鼠皮下注射乳腺癌MDA-MB-468细胞株,SK-BR-3组小鼠皮下注射乳腺癌SK-BR-3细胞株,每组40只.选取一半数量的小鼠,采用皮下注射法将细胞种植在裸鼠右侧腹股沟区,每天注射1次,连续注射4周后处死裸鼠,剥离瘤体,计算两组裸鼠皮下肿瘤的体积抑瘤率,取出两组小鼠模型中另一半小鼠,将阿霉素缓制剂注入两组小鼠模型皮下肿瘤旁,4周后处死两组小鼠,剥离瘤体,测量肿瘤大小.采用原位缺口末端标记法（TUNEL）法检测两组小鼠瘤体组织中肿瘤细胞的凋亡指数,观察阿霉素对两组乳腺癌细胞周期和凋亡的影响.结果 （1）肿瘤生长：至第8周时,两组小鼠肿瘤的体积分别为（3.212±0.151）和（2.924 ±0.316） cm3,两组间差异有统计学意义（t=2.758,P＜0.05）.（2）注射阿霉素后肿瘤体积抑制率结果：两组乳腺癌小鼠模型注射阿霉素缓释剂后,皮下瘤体停止生长并逐渐缩小,两组肿瘤体积差异有统计学意义（x2=2.53,P＜0.05）.（3）阿霉素对两组细胞周期和凋亡的影响：阿霉素使两组细胞生长阻滞在G0/G1期.阿霉素可显著引起MDA-MB-468细胞凋亡,最大凋亡率达62.5％.阿霉素处理SK-BR-3细胞后,亦可引起较明显的细胞凋亡,最大凋亡率可达53.8％.结论 成功制备两种乳腺癌转移瘤小鼠模型,同时制备阿霉素缓释剂,证实了其在乳腺癌转移瘤小鼠模型中抑制肿瘤生长的良好疗效.Objective To establish the tumor models of SK-BR-3 and MDA-MB-468 in subcutaneous xenograft,observe the two breast cancer cell lines in the establishment of tumor and the biological and morphological characteristics in to the subcutaneous transplantation tumor in nude mice.To judge the impact of biological function of adriamycin on two breast cancer cell subcutaneous implanted tumor in nude mice.Methods 80 nude mice were randomly divided into group MDA-MB-468 and group SK-BR-3,group MDA-MB-468 mice subcutaneous injection of breast cancer cells MDA-MB-468,SK-BR-3 mice by subcutaneous injection of breast cancer cell line SK-BR-3,with 40 rats in each group.Selection of half the number of mice by subcutaneous injection of the cells grown in nude mice right groin area,1 daily injections for 4 weeks after injection,mice were sacrificed and the tumor body.Two groups of nude mice tumor volume measurement,calculation of tumor volume inhibition rate,remove the other half mice in two groups in a mouse model of adriamycin,will slow preparation into two groups of mice subcutaneous tumor model by injection,daily 1 times,continuous injection for 4 weeks.Observation of two nude mice,the growth of the dietandmental status,tumor record,mice were killed after 4 weeks,the tumor volume,the tumor size was measured.The apoptosis index of tumor cells was detected by TdT-mediated dUTP nick end labeling （TUNEL） in two groups of mice tumor tissue,to observe the effect of adriamycin on the two groups of breast cancer cell cycle and apoptosis.Results （1） Tumor growth：at eighth weeks,two groups of mice tumor volume were （3.212 ±0.151） and （2.924 ±0.316） cm3,the difference was statistically significant between the two groups （t =2.758,P ＜ 0.05）.Results the tumor volume inhibition rate.（2） After adriamycin injection：two groups of mouse model of breast cancer after injection of adriamycin,subcutaneous tumors stopped growing and gradually narrowing,4 weeks later,the tumor volume was statistically significant differ

关 键 词：乳腺癌 种植瘤 生物学功能 

分 类 号：R730.5[医药卫生—肿瘤]