微小RNA-30a对胃癌细胞株SGC7901增殖凋亡及侵袭转移的影响  

Effect of microRNA-30a on proliferation, apoptosis, invasion and migration of human gastric carcinoma SGC7901 cells

作　　者：宋军[1] 樊瑞智 徐溢新[1] 宋虎[1] 付海啸[1] 白津 郑骏年 徐为[1] 

机构地区：[1]徐州医学院附属医院胃肠外科,江苏221002 [2]江苏省肿瘤生物治疗重点实验室

出　　处：《中华实验外科杂志》2015年第9期2160-2162,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省卫生厅面上科研项目（H201220）;江苏省“六大人才高峰”项目（2012-WS-068）;徐州市科技发展基金资助项目（KC134SH103）;江苏省第四期“333高层次人才培养工程”项目

摘　　要：目的 观察微小RNA-30a（miR-30a）对胃癌细胞生物学行为的影响.方法 将miR-30a模拟物及抑制物瞬时转染胃癌细胞株SGC7901,实验分未转染组、空白对照组、miR-30a模拟物转染组、miR-30a抑制物转染组4组,实时定量反转录聚合酶链反应（RT-qPCR）检测miR-30a的表达水平,细胞计数试剂盒（CCK-8）法检测细胞增殖能力,流式细胞术检测细胞凋亡及细胞周期,Transwell小室检测细胞体外侵袭迁移能力.结果 与两对照组比较,胃癌SGC7901细胞在转染模拟物后,miR-30a表达明显上调,而转染抑制物后miR-30a表达明显受抑,差异有统计学意义（P＜0.叭）.与对照组及抑制物转染组比较,miR-30a模拟物转染组细胞增殖活性、迁移和侵袭能力相对减弱（P＜0.05）,而细胞凋亡率[（32.1±2.1）％]较未转染组[（18.8±1.2）％]、空白对照组[（18.6±1.1）％]和抑制物转染组[（16.4±1.9）％]增加（P＜0.05）,细胞周期S期增多达（36.7±3.6）％,出现S期阻滞.结论 miR-30a可明显抑制胃癌细胞株SGC7901的增殖,促进其凋亡,抑制其迁移及侵袭能力.Objective To explore the effect of microRNA-30a （miR-30a） on the biological behaviors of human gastric carcinoma cells.Methods Experiments were divided into four groups：negative control group and blank control group,miR-30a mimics group,miR-30a inhibitor group.miR-30a mimics group was transfected with miR-30a mimics to enhance the functions of miR-30a,and miR-30a inhibitor group was transfected with miR-30a inhibitor.miR-30a mRNA expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction （RT-qPCR）.Cell proliferation was evaluated using the cell counting kit-8 （CCK-8） assay and apoptosis was assessed by flow cytometry.Invasion and migration were measured by Transwell chamber assays.Results The SGC7901 cells transfected with miR-30a mimics showed significantly higher miR-30a mRNA expression than the non-transfected SGC7901 cells and the transfected control cells （P ＜ 0.01）.The miR-30a mRNA expression was significantly lower in the SGC7901 cells transfected with the miR-30a inhibitor than the non-transfected SGC7901 cells and the transfected control cells （P ＜ 0.01）.The overexpression of miR-30a inhibited the viability and invasion and migration abilities,as shown in the cells transfected with the miR-30a mimics （P ＜ 0.05）.There was no significant difference between control group and blank group,and the apoptosis rate in the experimental group [（32.1 ± 2.1） ％] was significantly higher than that in the control group [（18.8 ± 1.2） ％],blank group [（18.6 ± 1.1） ％] and inhibitor group [（16.4 ± 1.9） ％] （P ＜ 0.05）,and the cell cycle was significantly arrested in S phase and the number of S phase cells was increased larger than （36.7 ± 3.6） ％ by flow cytometry.Conclusion miR-30a had an inhibitory effect on cell proliferation,induced apoptosis,and markedly inhibited invasion and migration of SGC7901 cells.

关 键 词：胃癌 微小RNA-30a 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]