癌症相关microRNA与靶基因的生物信息学分析  

Bioinformatic analysis of cancer-related microRNAs and their target genes

作　　者：周学[1] 杜宜兰 金萍[3] 马飞[3] 

机构地区：[1]南京师范大学泰州学院化学与生物工程学院,泰州225300 [2]第三军医大学大坪医院健康管理科,重庆400042 [3]南京师范大学生命科学学院,南京210046

出　　处：《遗传》2015年第9期855-864,共10页Hereditas（Beijing)

基　　金：南京师范大学泰州学院青年项目(编号:Q201206)资助

摘　　要：Micro RNAs(mi RNAs)是一类长度约为22nt的内源性非编码RNA,通过与靶基因转录本互补结合调控基因的表达。近年来,研究发现mi RNA与癌症发生密切相关,mi RNA可以直接充当癌基因或者抑癌基因而影响肿瘤的发生和生长。为更进一步揭示癌症相关mi RNA的特征及靶基因的功能,文章通过数据库搜索及文献检索,在人类基因组中发现了475个癌症相关mi RNA,系统地比较了癌症相关mi RNA与非癌症mi RNA以及基因内和基因间区癌症相关mi RNA在保守性、SNP位点分布、癌谱及转录调控等特性。研究发现,癌症相关mi RNA比非癌症mi RNA保守性要强,发生SNP概率比较低,同时发现mi RNA所涉及癌症数目与保守性成正相关。基因组定位分析发现,癌症相关mi RNA比非癌症mi RNA更倾向于成簇存在。进一步对宿主基因、癌症相关mi RNA及作用的靶基因与癌症发生进行关联分析,发现一些非癌症mi RNA的宿主基因倾向于被癌症mi RNA作用。本研究结果为深入理解mi RNA与癌症之间的关系,以及进一步为mi RNA作为癌症诊断指示物提供理论依据。MicroRNAs （miRNAs） are a class of --22 nucleotide endogenous noncoding RNAs which regulate gene expression by targeting complementary transcripts. Recent studies have found that miRNAs are closely related to tumorigenesis and can act as oncogenes or tumor suppressor genes to influence the occurrence and development of tumor. To further reveal characteristics of cancer-related miRNAs and the functions of miRNA targets, we obtained 475 miRNAs involved in cancer through database searching and information retrieval. We systematically analyzed and compared thefeatures including conservation, SNP distribution, cancer spectrum width, and transcriptional regulation between cancer and non-cancer related miRNAs as well as between intergenic and intragenic miRNAs. Our results showed that cancer-related miRNAs have higher conservation and lower SNP frequency compared to non-cancer-related miRNAs, and the cancer spectrum of one miRNA is positively correlated with its conservation. Genome analysis showed that cancer-related miRNAs tend to present as clusters compared with non-cancer-related miRNAs. Further association analysis between cancer progression and host genes, cancer-related miRNAs or target genes found that the host genes of some non-cancer related miRNAs tend to be targeted by cancer-related miRNAs. This study provides theoretical basis for further understanding the relationship between miRNA and cancer progression as well as the miRNA-based cancer diagnosis.

关 键 词：MicroRNAs(miRNAs) 癌症 靶基因 宿主基因 生物信息学 

分 类 号：R730.2[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]