培养不同时间CIK细胞免疫表型变化与其抗肿瘤活性关系的研究  

Study of relationship of CIK cells phenotype change and its anti-tumor activity at different time

作　　者：赵鑫[1] 常颖[1] 刘玉侠[2] 卢卫平[1] 王哲[1] 王宝[1] 于鸿[2] 王启文[1] 

机构地区：[1]吉林省肿瘤医院,吉林长春130012 [2]吉林省肿瘤防治研究所,吉林长春130012

出　　处：《中国实验诊断学》2015年第9期1455-1457,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨培养不同时间的CIK(cytokine induced killer cells)细胞免疫表型变化及其对肿瘤细胞杀伤活性的关系,为CIK临床治疗肿瘤提供实验数据。方法分离人外周血单核细胞,加IFNγ、IL1α、CD3Ab、IL-2等细胞因子诱导CIK细胞并进行鉴定,将经过鉴定的CIK与肺癌细胞A549共同培养,检测不同时间培养的CIK表型变化与对A549的体外杀伤效果的关系。结果培养不同时间(10天、15天、20天)的CIK细胞CD3+CD56+CD16+表达率分别为42.1%,63.3%,77.1%,对A549的杀伤效率分别为41.75%,54.43%,79.31%。结论随着培养时间延长,CIK免疫表型表达率逐渐上升,对A549的杀伤效率也随之逐渐增强,本实验杀伤活性最高为20天,提示在临床应用时可以根据不同时间CIK免疫表型表达情况选择应用时间,达到使用的最佳效果。Objective To Explore the relationship of CIK cells（cytokine induced killer cells）phenotype change and its anti-tumor activity at different time,provide experimental data for the clinical application of CIK.Methods Isolated from human peripheral blood mononuclear cells,Plus IFNγ,IL1α,CD3Ab,IL-2 and other cytokines induced CIK cells and identified,then,identified CIK with A549 cells were co-cultured,detect different time cultured CIK immune pheno-typic changes and on A549 cells killing effects in vitro.Results Culture at different times （10 days,15 days,20 days） of CIK cells CD3 ＋ CD56 ＋ CD16 ＋ expression rates were 42.1%,63.3%,77.1%,Cytotoxicity against A549 were 41.75%,54.43%,79.31%.Conclusion As the incubation time,CIK phenotype expression rate gradually increased, Killing efficiency of A549 with the gradual enhancement,In this study,the cytotoxic activity of up to 20 days,prompt that we can depending on the CIK immune phenotype expression choose to apply the time,achieve the best results.

关 键 词：CIK细胞 细胞表型 肺癌细胞A549 杀伤活性 

分 类 号：R730.51[医药卫生—肿瘤]