重组人血管内皮抑制素对小鼠胃癌c-Myc,bFGF表达的影响  

Effect of Recombinant Human Endostatin on Expression of c-Myc and bFGF in Gastric Cancer

作　　者：郭志远[1] 付吕平[1] 付志国[1] 康振桥[1] 祁卫华[1] 

机构地区：[1]邯郸市中心医院放疗二科,河北邯郸056001

出　　处：《标记免疫分析与临床》2015年第9期916-920,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的通过构建荷胃癌小鼠模型研究重组人血管内皮抑制素对癌基因c-Myc和成纤维细胞生长因子(bFGF)表达的影响及可能机制。方法 20只小鼠皮下注射MFC小鼠前胃癌细胞从而构建小鼠胃癌移植瘤模型,后随机分成两组,每组10只,观察组腹腔注射0.8mg/kg重组人血管内皮抑素,对照组腹腔注射等量生理盐水。每天观察小鼠的一般情况,每3天测量肿瘤体积,绘制肿瘤体积生长曲线并对两实验组肿瘤体积进行比较;应用RT-PCR、Western bolt及免疫组织化学法检测两组小鼠肿瘤组织中c-Myc和bFGF的表达,免疫组织化学染色法检测肿瘤组织微血管密度(MVD)的表达,并通过Spearman分析c-Myc和bFGF两者的相关性。结果在用药的过程中,对照组小鼠随着移植瘤结节的增大而发生行为明显改变,但观察组小鼠精神、反应、活动及进食饮水均如初,未见明显不良反应。治疗后第9 d开始,观察组小鼠移植瘤体积明显小于对照组(P<0.05),在第21 d最明显(P<0.01)。RT-PCR检测显示c-Myc,bFGF mRNA相对表达量明显低于对照组(P<0.05);Western blot检测显也显示观察组c-Myc,bFGF蛋白的表达量明显低于对照组(P<0.05);免疫组织化学检测显示c-Myc,bFGF阳性细胞数均明显低于对照组(P<0.05),c-Myc,bFGF平均灰度值明显高于对照组(P<0.05);对照组平均MVD为9.2±1.3,观察组为2.9±1.0,两组之间差异亦具有统计学差异(P<0.05)。且Spearman分析得出小鼠胃移植瘤中c-Myc和bFGF mRNA存在正相关(r=0.853,P=0.000)。结论重组人血管内皮抑制素通过抑制胃癌组织c-Myc,bFGF基因表达和血管生成。Objective To explore the effect of recombinant human endostatin on the expression of c-Myc and fibroblast growth factor （bFGF） and its possible mechanism by constructing a model of gastric cancer xenograft in mice. Methods 20 mice were injected subcutaneously MFC to establish gastric cancer xenograft model, and then randomly divided into two groups. 0.8mg/kg recombinant human endostatin was injected intraperitoneally into mice model in observation group, and the control group was injected with the same amount of saline. The general condition of mice was observed every day, and the tumor volume was measured every 3 days. Expression of bFGF and c- Myc mRNA and protein in tumor tissues of the two groups were detected by RT-PCR, Western bolt and immunohistochemistry,respectively. The expression of microvessel density （MVD） in tumor tissue was detected by immunohistochemical staining. Results The behavior of mice in control group had an obviously changes as transplanted tumor nodules increased, but no obvious adverse reaction and changes in observation group on the mice spirit, reaction, activity, eating and drinking water. The tumor volume in observation group after 9 days of treatment was significantly smaller than that of the control group （ P 〈 0. 05 ）. The relative expression of c-Myc, bFGF mRNA and hFGF protein in observation group were significantly lower than that of the control group （P 〈 0.05 ）. The c-Myc and bFGF positive cells in observation group were significantly lower than the control group （P 〈 0.05 ）, the average gray value of c- Myc and bFGF was significantly higher than that of the control group （ P 〈 0.05 ）. The average MVD of in control group and observation group were 9. 2 ± 1.3, and 2.9 ± 1.0 with statistically significance （ P 〈 0.05 ）. There was positive correlation between bFGF and c-Myc mRNA in the transplanted gastric tumor of mice （r =0. 853 ,P = 0. 000）. Conclusion Recombinant human endostatin could inhibit the expression of c-Myc, bFGF and ang

关 键 词：重组人血管内皮抑制素 胃肿瘤 血管生成 C-MYC BFGF 

分 类 号：R735.2[医药卫生—肿瘤]