异硫氰酸荧光素染色在真菌性角膜炎中的应用  

Application of fluorescein isothiocyanate staining in fungal keratitis

作　　者：阎艳[1] 武鹏安 刘先宁 王东岳[1] 张仙娇[1] 崔家瑞[1] 

机构地区：[1]中国陕西省西安市第一医院病理科,710002 [2]中国陕西省西安市第一医院陕西省眼科研究所,710002

出　　处：《国际眼科杂志》2015年第10期1800-1801,共2页International Eye Science

摘　　要：目的:寻找真菌性角膜炎组织切片快速、准确、方便操作的特殊染色方式。方法:采用经真菌培养均为阳性的真菌性角膜炎患者的角膜标本38例,常规固定脱水透明浸蜡,进行4μm石蜡切片,脱蜡至水,选用异硫氰酸荧光素(FITC)荧光法和过碘酸雪夫氏染色法(PAS)进行染色对比。结果:异硫氰酸荧光素法检测阳性率为97.4%(37/38),过碘酸雪夫氏染色法检测阳性率为86.8%(33/38)。结果比较有统计学差异(P<0.001)。结论:异硫氰酸荧光素法镜检真菌性角膜炎优于过碘酸雪夫氏染色法镜检。AIM： To find special staining methods which is fast, accurate and easy to operate for fungal keratitis tissue section. METHODS：Thirty- eight cases with fungal keratitis were diagnosed by positive fungal culture. Corneal specimens of these cases were conventionally fixed, dehydrated and dipped in wax for 4μm paraffin sections, then subject to conventional dewaxing to water. The results of fluorescein isothiocyanate （ FITC） and periodic acid-Schiff （ PAS） staining were compared. RESULTS： Positive detection rate of FITC staining was 97. 4% （37/38）, positive detection rate of PAS staining was 86. 8% （ 33/38 ）, with statistically significant difference （P〈0. 001）. CONCLUSION： FITC staining method is simple, fast, accurate, andhas high positive rate. It is superior to PAS staining, and is worth of promoting.

关 键 词：真菌性角膜炎 石蜡切片 异硫氰酸荧光素染色法 过碘酸雪夫氏染色法 

分 类 号：R772.21[医药卫生—眼科]