几个转bar基因水稻外源基因的遗传学行为研究  

Genetic Mode of bar Gene in Transgenic Rice

作　　者：吴明国[1] 华志华[1] 林建荣[1] 黄大年[1] 

机构地区：[1]中国水稻研究所,浙江杭州310006

出　　处：《中国水稻科学》2002年第2期111-114,共4页Chinese Journal of Rice Science

基　　金：浙江省"九五"生物技术重点项目 (96110 2 2 16)

摘　　要：利用春江 0 3等 4个品种的转基因株及后代稳定系进行遗传研究表明 ,bar基因作为一个显性单位已整合在水稻核基因组中 ,转基因株稳定系 TR3、TR4、TR5、TR6对除草剂 Basta的抗性均受 1对显性基因控制 ,且 4个转基因株稳定系间 bar基因互不等位 ,不同位点的 bar基因之间存在基因互作 ,表现为基因重叠作用。Southern杂交分析结果表明 ,TR5、TR6的 bar基因整合位点互不相同 ,但可通过杂交育种重组到同一个水稻核基因组中。The inheritance study of transgenic plants from four varieties and their stable lines demonstrated that bar gene was integrated into rice nucleus genome as a dominant gene. The herbicide resistance of transgenic stable lines TR3, TR4, TR5 and TR6 was controlled by a pair of dominant genes, and the bar gene of the four transgenic stable lines is non allelic one another. The bar genes at different loci have gene interaction, which behaving as gene overlapping action. Result of Southern analysis indicated that the bar gene of TR5 and TR6 is non allelic, and the bar gene at different loci could be recombined into the same rice nucleus genome by cross breeding.

关 键 词：外源基因 遗传学行为 转基因 水稻 BAR基因 

分 类 号：S511[农业科学—作物学] S188