MCI-186对癫痫大鼠海马组织中细胞色素C表达的影响  

The effect of MCI-186 on the expression of cytochrome C in hippocampus of epilepsy rats

作　　者：余汉忠 郝凌云[3] 魏学文[2] 马萍[1] 袁玉玶 

机构地区：[1]徐州医学院医学技术学院,江苏徐州221002 [2]江苏省徐州市第一人民医院检验科,221002 [3]徐州医学院麻醉学教研室,江苏徐州221002

出　　处：《蚌埠医学院学报》2015年第9期1155-1157,共3页Journal of Bengbu Medical College

基　　金：江苏大学医学临床科技发展基金项目(JLY20140126)

摘　　要：目的:探讨MCI-186对海人酸(KA)诱导癫痫大鼠海马组织细胞色素C(Cyt C)表达的影响。方法:SD大鼠随机分为癫痫对照组、癫痫组、药物对照组组和MCI-186组。采用脑室注射KA制作大鼠癫痫模型。应用Western-blot法检测细胞质和线粒体中Cyt C。结果:癫痫组和药物对照组Cyt C在线粒体的表达均较MCI-186组减少(P<0.01),癫痫组和药物对照组Cyt C在细胞质的表达均较MCI-186组增加(P<0.01)。结论:MCI-186可以减少KA诱导Cyt C由线粒体向细胞质的释放,通过抑制线粒体凋亡通路对海马细胞发挥保护作用。Objective：To investigate the effects of MCI-186 on the expression of cytochrome C（Cyt C） in hippocampus of epilepsy rats induced by kainic acid（ KA） . Methods：The SD rats were randomly divided into the control group,seizure group,drug control group and MCI-186 group. The seizures model of rats were established by injecting KA into encephalocoele. The Cyt C expressions in cytoplasm and mitochondria were detected using Western-blot. Results：The Cyt C expressions in mitochondria in seizure group and drug control group were lower than that in MCI-186 group（P〈0. 01）. The Cyt C expressions in cytoplasm in seizure group and drug control group were higher than that in MCI-186 group（P〈0. 01）. Conclusions：MCI-186 may decrease the release of Cyt C from mitochondria to cytoplasm,and which can protect the hippocampus by inhibiting the mitochondrial apoptotic pathway.

关 键 词：癫痫 MCI-186 海人酸 细胞色素C 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]