高效液相色谱-串联质谱法检测塑料类食品接触材料中9种紫外吸收剂的特定总迁移量  

Simultaneous Determination of Total Specific Migration Limit of 9 Types of Ultraviolet Absorbers in Food Simulants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：肖晓峰[1] 王建玲[1] 陈彤[1] 刘艇飞 何军[1] 邓弘毅 杨娟娟[1] 

机构地区：[1]台州出入境检验检疫局,浙江台州318000

出　　处：《分析测试学报》2015年第9期1021-1026,共6页Journal of Instrumental Analysis

基　　金：国家质检总局科技计划项目资助课题(2013IK022)

摘　　要：建立了高效液相色谱-串联质谱（HPLC-MS/MS）同时测定塑料制品食品模拟物（10%乙醇、20%乙醇、50%乙醇、3%乙酸和橄榄油）浸泡液中UV-24,UV-0,UV-9,DHBP,UV-531,HHBP,UV-P,UV-327和UV-326共9种紫外吸收剂的特定总迁移量的方法。以食品模拟物浸泡待测样品,冷却至室温并混匀,水基食品模拟物（10%乙醇、3%乙酸、20%乙醇、50%乙醇）用乙醇1∶10稀释,经亲水性聚四氟乙烯（PTFE）针头过滤器过滤后进样;橄榄油用乙腈正庚烷提取后,下清液用PTFE过滤器过滤后进样。采用C8柱梯度洗脱分离;质谱采用电喷雾离子源,在负离子多反应监测（MRM）模式下运行。方法的线性关系良好（r≥0.995 4）,在5种食品模拟物中的定量下限为0.01~0.30 mg/kg;1~35 mg/kg加标水平下的回收率为80.0%~127%,相对标准偏差（RSD）为0.8%~8.0%。该方法的色谱分离效果和线性关系较好,回收率和准确度高,可用于实际样品的检测。A novel method for the simultaneous determination of total specific migration limit of UV-24,UV- 0,UV- 9,DHBP,UV- 531,HHBP,UV- P,UV- 327 and UV- 326 in food simulants（ 10% ethanol,20% ethanol,50% ethanol,3% acetic acid and olive oil） was developed using high performance liquid chromatography- tandem mass spectrometry（ HPLC- MS/MS）. After the migration test,the soaking solution was cooled down,and homogenized. Olive oil food simulant was extracted with acetonitrile and n-heptane,the clear acetonitrile solution and other aqueous food stimulant diluted with ethanol（ 1 ∶ 10） were filtered through a hydrophilic polytetrafluoroethylene filter with a disposable syringe before injection. The Sun FireTMC8 column and gradient elution were selected to separate. The AB Qtrap 4500 tandem quadrupole mass spectrometer was operated under multiple reaction monitoring（ MRM） and ESI-mode. The limits of quantitation were between 0. 01 mg/kg and 0. 30 mg/kg. The linearity of the method was good with correlation coefficients（ r） not less than0. 995 4 over the certain concentration range. The recoveries at spiked concentration levels of 1- 35 mg/kg were between 80. 0% and 127% with relative standard deviations of 0. 8%- 8. 0%. With the high sensitivities,good recoveries and accuracies,the method could be applied for the analysis of food contact material samples.

关 键 词：高效液相色谱-串联质谱(HPLC-MS/MS) 紫外吸收剂 特定总迁移量 食品模拟物 

分 类 号：O657.63[理学—分析化学] TQ314.257[理学—化学]