二氢杨梅素对肝癌细胞黏附、侵袭及迁移的抑制作用及机制  

Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism

作　　者：邱志东 罗芳兰[2] 舒洋[2] 温小军[2] 张庆余[3] 廖雅琳 黎然[3] 缪辉来[3] 

机构地区：[1]广东省深圳市第七人民医院普通外科,广东深圳518000 [2]广东医学院研究生学院,广东湛江524000 [3]广东医学院附属医院肝胆外科,广东湛江524000 [4]广东医学院第一临床学院,广东湛江524000

出　　处：《中国普通外科杂志》2015年第9期1263-1268,共6页China Journal of General Surgery

摘　　要：目的:研究二氢杨梅素(DHM)对肝癌细胞黏附、侵袭和迁移能力的影响及其可能机制。方法:用不同浓度DHM处理肝癌MHCC97L细胞后,分别检测细胞的黏附能力、迁移与侵袭能力,以及E-cadherin、MMP-2、MMP-9和VEGF蛋白表达。结果:与空白对照细胞比较,DHM处理后的MHCC97L细胞黏附力明显降低、侵袭与迁移力明显减弱(均P<0.05);E-cadherin表达明显上调,而MMP-9、VEGF蛋白表达明显下调的水平(均P<0.05),但MMP-2蛋白的表达无明显改变(均P>0.05)。结论:DHM可能通过调控E-cadherin、MMP-9和VEGF蛋白的表达抑制肝癌细胞的黏附、迁移和侵袭。Objective： To investigate the effect of dihydromyricetin （DHM） on adhesion, invasion and migration abilities of hepatocellular carcinoma （HCC） cells and the possible mechanisms. Methods： HCC MHCC97L cells were exposed to different concentrations of DHM, and then, the adhesion, invasion and migration abilities of the cells were examined, and the protein expressions of E-cadherin, MMP-2, MMP-9 and VEGF were also determined. Results： In MHCC97L cells treated with DHM compared with blank control cells, the abilities of adhesion, invasion and migration were all significantly deceased （all P〈0.05）, the E-cadherin expression was upregulated, and the MMP-9 and VEGF expressions were downregulated significantly, but the MMP-2 expression showed no significant change （P〉0.05）. Conclusion： DHM can inhibit the adhesion, invasion and migration of HCC cells by regulation of E-cadherin, MMP-9 and VEGF expressions.

关 键 词：癌 肝细胞 二氢杨梅素 肿瘤侵润 

分 类 号：R735.7[医药卫生—肿瘤]