子宫颈鳞状细胞癌中Galectin-7表达与HPV16感染的相关性  

Expression of Galectin-7 in squamous cell carcinoma of the cervix and its association with HPV 16 infection

作　　者：贾索尔·肖克热提 阿力甫江·海力力 阿力木·伊力亚斯 海米提·阿布都力木[2] 

机构地区：[1]新疆医科大学临床医学院2010临床医学七年制,乌鲁木齐830011 [2]新疆医科大学组胚教研室,乌鲁木齐830011

出　　处：《临床与实验病理学杂志》2015年第9期1001-1004,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家级大学生创新项目(CX2013012)

摘　　要：目的探讨半乳凝素-7(Galectin-7)在维吾尔族与汉族妇女子宫颈鳞状细胞癌和子宫颈炎中的表达,并分析其与HPV 16感染、子宫颈癌分化程度、患病年龄的相关性。方法采用免疫组化SP法分别检测Galectin-7在维吾尔族21例子宫颈炎和49例子宫颈鳞状细胞癌、汉族25例子宫颈炎和29例子宫颈鳞状细胞癌中的表达;采用PCR技术检测HPV 16感染情况。利用图像分析软件Image-Pro Plus(IPP)6.0半定量检测子宫颈病变中Galectin-7的累计光密度(integrated optical density,IOD)值。结果 PCR检测显示维吾尔族子宫颈鳞状细胞癌中HPV 16阳性者20例,阴性者29例;子宫颈炎中HPV 16阳性者5例,阴性者16例。汉族子宫颈癌中HPV 16阳性者2例,阴性者27例;子宫颈炎中HPV 16均阴性。Galectin-7在维吾尔族子宫颈鳞状细胞癌中的表达低于子宫颈炎组织(P<0.05),其在维吾尔族HPV 16阳性子宫颈鳞状细胞癌组织中的表达低于HPV 16阴性组织(P<0.05)。Galectin-7表达与子宫颈鳞状细胞癌分化程度、患病年龄无相关性(P>0.05)。结论维吾尔族子宫颈鳞状细胞癌与子宫颈炎组织相比,Galectin-7表达量下降;另外其在维吾尔族HPV 16阳性子宫颈鳞状细胞癌中的表达低于HPV 16阴性者,这可能与子宫颈鳞状细胞癌的发生、发展、浸润、转移密切相关,检测其表达对维吾尔族妇女子宫颈癌诊断有一定的参考价值。Purpose To study the expression of Galectin-7 in squamous carcinoma of the cervix and its correlation with HPV 16 infection in Uyghur and Han women. Methods The expression of Galectin-7 in paraffin-embedded cervical tissues collected from Uyghur women including 21 patients with cervicitis and 49 patients with squamous carcinoma of the cervix and Han women including 25 patients with cervicitis and 29 patients with squamous cervical cancer was evaluated by immunohistochemical SP method. The infection of HPV 16 was evaluated by PCR amplification of HPV DNA from the corresponding cervical tissue samples. The expression of levels of Galectin-7 in cervical tissues was analyzed according to the corresponding integrated optical density （ IOD） value of Galectin-7 in cervical lesions with professional image analysis software Image-Pro Plus （IPP） 6. 0. Results PCR detection of HPV 16 in Uyghur patients from Kashghar showed that 20 patients were positive and 29 cases were negative for HPV 16 in squamous cervical cancer patients whereas in patients with cervicitis, only 5 cases were positive, 16 cases were negative for HPV 16. Furthermore the detection of HPV 16 in Han patients showed that 2 cases were positive and 27 cases was negative in patients with squamous cervical cancer patients, and all the cases were negative for HPV 16 in patients with cervicitis. The expression of Galectin-7 in squamous cervical cancer tissues was signifi-cantly lower than that in the cervicitis patients （P〈0. 05）. The expression of Galectin-7 in HPV 16 positive squamous cervical cancer patients was significantly lower than that in HPV 16 negative patients （ P〈0. 05 ） . There was no obvious correlation between the expression levels of Galectin-7 in squamous cervical cancer patients with the differentiation degree and disease oneset age both in Uyghur and Han patients. Conclusion Compared with cervicitis tissues, Galectin-7 expression in squamous cervical cancer is down-regulated in Uyghur women from Kashghar region implying tha

关 键 词：子宫颈肿瘤 鳞状细胞癌 Galectin-7 HPV16 分化程度 发病年龄 

分 类 号：R737.33[医药卫生—肿瘤]