miR-200a、PTEN在结直肠癌中的表达及其临床意义  

Expression of miR-200a and PTEN in colorectal carcinoma and their clinical significance

作　　者：孙晶璐 李昊[1] 尹玉[1] 李懿君[1] 王弦[1] 黄山[1] 江燕[1] 詹鹤琴[1] 杨枫[1] 

机构地区：[1]安徽医科大学病理学科,合肥230032 [2]安徽医科大学第四附属医院病理科,合肥230022

出　　处：《临床与实验病理学杂志》2015年第9期1005-1008,1012,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81201536);安徽省高等学校自然科学基金(KJ2011A160)

摘　　要：目的探讨miR-200a与PTEN在结直肠癌中的表达及其与临床病理特征的关系。方法收集结直肠癌手术标本87例以及距癌灶边缘5 cm以上切缘的正常结直肠黏膜作为对照,应用原位分子杂交和免疫组化En Vision两步法检测结直肠癌及癌旁组织中miR-200a与PTEN的表达,分析二者与结直肠癌临床病理特征的关系。结果原位分子杂交结果显示,结直肠癌组织中miR-200a的阳性率明显高于癌旁对照组(P<0.01),miR-200a表达与肿瘤分化程度相关(r_s=0.503,P<0.01)。免疫组化结果显示,结直肠癌组织中PTEN的阳性率明显低于癌旁对照组(P<0.01),PTEN表达与肿瘤分化程度有相关性(r_s=-0.493,P<0.01)。二者在结直肠癌组织中的表达均与患者年龄、性别、肿瘤大小、浸润深度、淋巴结转移及TNM分期等无显著相关性(P>0.05)。相关性分析显示,结直肠癌中miR-200a与PTEN的表达呈显著负相关(P<0.01)。结论高表达的miR-200a通过靶向抑制PTEN的表达参与结直肠癌的发生、发展,miR-200a与PTEN有望成为结直肠癌早期诊断、治疗、预后判断的潜在生物学指标。Purpose To investigate the expression of miR-200a and PTEN in colorectal carcinoma （CRC） and their relationships with clinicopathologic features. Methods In situ hybridization and immunohistochemistry （ EnVision method） for miR-200a and PTEN were performed in 87 CRCs and normal colorectal tissues distant from tumors. Relationship between expression of miR-200a and PTEN and clinicopathologic parameters of CRC was also analyzed. Results The in situ hybridization showed that the positive expression rate of the miR-200a in CRC was higher than those in normal colorectal mucosa （P〈0. 01）. The expression of miR-200a was correlated with the degree of tumor differentiation （rs =0. 503, P〈0. 01）. The immunohistochemistry showed that the positive expression rate of the PTEN in CRC was lower than those in normal colorectal mucosa （P〈0. 01）. The expression of miR-200a was correlated with the degree of tumor differentiation （rs = -0. 493, P〈0. 01）. Expression of miR-200a and PTEN was not correlated with age, sex, tumor size, depth of tumor invasion, lymph node metastasis and TNM stage （P〉0. 05）. The expression of miR-200a had a close negative correlation to that of PTEN in CRC （P〈0. 01）. Conclusions Overexpression of miR-200a might be associated with the occurrence and development by targeting PTEN, and they could be the indicators in the early diagnosis,treatment and prognosis of CRC.

关 键 词：结直肠肿瘤 原位分子杂交 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]