结肠癌组织中OTUB1的表达及其临床意义  

Expression of OTUB1 in colon cancer and its clinical significance

作　　者：姜维娜[1] 黄维清[1] 刘鑫[1] 刘红云[1] 孔庆暖[1] 韩增磊[1] 陈桦[1] 

机构地区：[1]青岛市市立医院病理科,266071

出　　处：《临床与实验病理学杂志》2015年第9期1009-1012,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：青岛市产学研合作计划[12-1-4-16-(3)-jch]

摘　　要：目的探讨OTUB1在结肠癌组织中的表达及其临床意义。方法采用实时定量PCR和免疫组化SP法检测OTUB1在结肠癌组织和癌旁组织中的表达。结果 OTUB1 mRNA在结肠癌组织中的表达较癌旁组织高3.5倍,癌组织中OTUB1的蛋白表达水平也明显高于癌旁组织(P<0.05),且呈现癌旁组织、腺瘤、结肠癌逐渐递增的趋势(P<0.05)。OTUB1高表达与肿瘤大小、分化程度和淋巴结转移密切相关。结论 OTUB1在结肠癌的发生、发展过程中发挥重要作用。Purpose To investigate the expression of OTUB1 in colon cancer and the relationship between its expression and some clinicopathologic parameters. Methods Quantitative real-time PCR and immunohistochemical SP method were carried out in selected colon cancer and normal mucosa tissues. Results OTUB1 mRNA in colon cancer was 3. 5-fold higher than the normal mucosa. The expression of OTUB1 protein in the colon cancer was significantly higher than normal mucosa （P〈0. 05）. Moreover, its expression in normal tissues, adenoma and colon cancer showed a gradually increasing trend （P〈0. 05）. The higher expression of OTUB1 in colon cancer was related with tumor size, differentiation and lymph node metastasis. Conclusions OTUB1 may play an important role in colon cancer development.

关 键 词：结肠肿瘤 OTUB1 实时定量PCR 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]