EGCG联合顺铂对乏氧人肺腺癌A549细胞凋亡的促进作用  

Effect of EGCG in combination with cisplatin on apoptosis of human lung adenocarcinoma A549 cells under hypoxia conditions

作　　者：李芳[1,2] 黄瓅[2] 苏晓丽[2] 胡成平[2] 

机构地区：[1]郑州大学第一附属医院呼吸与危重症医学科,河南郑州450052 [2]中南大学湘雅医院呼吸与危重症医学科,湖南长沙410008

出　　处：《河南医学研究》2015年第8期1-5,共5页Henan Medical Research

摘　　要：目的探讨EGCG联合顺铂对乏氧人肺腺癌A549细胞凋亡的影响。方法采用氯化钴(Co Cl2)建立人肺腺癌A549细胞体外化学乏氧模型,分为乏氧组、EGCG+乏氧组及常氧组。MTT法、光镜下Hoechst染色法、Annexin VFITC/PI双染法测定EGCG单独或联合顺铂时对乏氧A549细胞生长、细胞核形态及凋亡率的影响。结果 200μmol/L Co Cl2乏氧条件下,加入0、25、50、100、150、200 mg/L EGCG作用48 h后,以50 mg/L EGCG为拐点,A549细胞生长抑制率呈EGCG浓度依赖性增加。与0 mg/L EGCG相比,50 mg/L EGCG作用时,部分乏氧A549细胞核形态不规则,染色质固缩、致密浓染,凋亡率由(0.41%±0.14%)增加至(3.15%±0.28%)(P<0.05);顺铂IC50由(107.01±10.19)μmol/L降低至(41.28±3.85)μmol/L(P<0.05);与10μmol/L顺铂联合时,A549细胞凋亡率由(8.12%±0.67%)增加至(13.28%±3.11%)(P<0.05)。结论 EGCG联合顺铂对乏氧A549细胞的凋亡起促进作用。Objective To investigate the effect of EGCG in combination with cisplatin on apoptosis of human lung adenocarci-noma A549 cells under hypoxia conditions. Methods Cobalt chloride（CoCl2 ）was used to establish chemical hypoxia model of A549 cell in vitro. Proliferation,nuclear morphology and apoptosis rate of A549 cell under EGCG alone or in combination with cisplatin were measured by MTT,Hoechst staining and AnnexinV - FITC / PI method,respectively. Results after cultured in 0,25,50,100,150,200 mg/ L EGCG for 48 hours,growth inhibition rates of hypoxic A549 cell increased in a EGCG dose de-pendent manner,with 50 mg/ L as the inflection point. Compared with the 0 mg/ L EGCG,50 mg / L EGCG made the nuclear of some A549 irregular with chromatin pyknosis and condensed,accompanied apoptotic rate increased to（3. 15% ± 0. 28% ） from（0. 41% ± 0. 14% ）（P〈 0. 05）,IC50 of cisplatin decreased from（107. 01 ± 10. 19）μmol/ L to（41. 28 ± 3. 85）μmol/ L （P〈 0. 05）,when combined with 10 μmol/ L cisplatin,the apoptotic rate increased to（13. 28% ± 3. 11% ）from（8. 12% ± 0. 67% ）（P〈 0. 05）. Conclusion EGCG in combination with cisplatin could promote the apoptosis rate of A549 cell under hy-poxic condition.

关 键 词：EGCG 乏氧 肺腺癌 顺铂 

分 类 号：R734.2[医药卫生—肿瘤]