见血青总皂苷体外溶血与抗氧化活性研究  

Hemolytic and antioxidant activities in vitro of total saponins from Liparis nervosa

作　　者：曾春菡[1] 刘小波[1] 曹弈璘[1] 王丽[1] 

机构地区：[1]四川大学生命科学学院,成都610065

出　　处：《四川大学学报（自然科学版）》2015年第5期1141-1144,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家科技基础性工作专项重点项目(2008FY110400)

摘　　要：利用溶血与凝聚检查法检测见血青总皂苷体外溶血活性,以及用DPPH自由基法、水杨酸法、邻苯三酚自氧化法研究其体外抗氧化活性.实验结果显示：总皂苷在0.12-0.6mg/mL范围内未表现出溶血作用,但出现了红细胞凝聚现象;对DPPH自由基的清除率与BHT相当,其IC50值为0.17mg/mL;对羟自由基、超氧阴离子自由基的清除率较Vc弱,且在0.025-0.475mg/mL浓度范围内变化不大.Hemolytic activity of total saponins from Liparis nervosa was detected by hemolysis and bloodcell agglutination test, and the antioxidant activity was evaluated by inhibiting the formation of DPPHradical, hydroxyl radical and superoxide anion radical. The results showed that the total saponins had nohemolysis effect in the range of 0.12-0.6 mg/mL, while blood cell agglutination was observed. Thescavenging rate to DPPH radical was equivalent to that of BHT, which the ICso value was 0.17 mg/mL,and that of hydroxyl radical and superoxide radical were weaker than Ve in the range of 0. 025-0. 475mg/mL.

关 键 词：见血青 总皂苷 溶血 抗氧化活性 

分 类 号：Q946.83[生物学—植物学]