microRNA-218下调survivin表达的抗食管癌作用  

Anti-cancer effect of microRNA-218 plays an by targeting survivin in esophageal carcinoma tissues

作　　者：万军[1] 车云[1] 康宁宁[1] 张仁泉[1] 

机构地区：[1]安徽医科大学第一附属医院普胸外科,安徽合肥230022

出　　处：《中华肿瘤防治杂志》2015年第17期1378-1381,1387,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81302028);安徽省自然科学基金(1208085QH159)

摘　　要：目的探讨微小RNA218(microRNA-218,miR-218)在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达及其抗肿瘤的作用和分子机制。方法收集2014-07-03-2015-03-15安徽医科大学第一附属医院普胸外科行手术切除的食管癌及对应癌旁组织50例,qRT-PCR技术分析miR-218在ESCC组织及癌旁组织中的表达,统计分析miR-218表达水平与患者临床病理特征的相关性;免疫组化染色检测ESCC组织中Survivin蛋白的表达,Spearman相关性分析survivin蛋白与miR-218表达的相关性;在食管癌TE-1细胞中过表达miR-218,分别通过qRT-PCR及蛋白质印迹检测转染miR-218后survivin mRNA及蛋白的表达水平变化。结果 miR-218在食管癌组织中表达水平为4.392±0.203,显著低于对应癌旁组织的9.301±0.211,P<0.001;低表达状态的miR-218与肿瘤直径增大(>5cm;t=2.872,P=0.006)及高TNM分期(Ⅲ+Ⅳ期;t=3.231,P=0.003)显著相关;在食管癌组织中,miR-218与其下游潜在靶点survivin的表达呈显著负相关关系,P<0.05。miR-218转染人食管癌TE-1细胞后,可显著降低细胞内survivin mRNA及蛋白的表达水平,P<0.05。结论 miR-218在食管癌组织中呈现低表达状态并与肿瘤不良临床病理特征有关。miR-218可能是通过下调survivin的表达来发挥其抗肿瘤效应。OBJECTIVE To investigate the expression and the the anti-cancer mechanisms of microRNA 218 in E sophageal squamous cell carcinoma（ESCC）. METHODS Fifty ESCC tissues and matched tumor-adjacent tissues were collected from thoracic surgery,department of the first affiliated hospital of Anhui Medical University from July 3, 2014 to March 15, 2015. qRT-PCR was applied to detect the relative expression of miR-218 in ESCC tissues compared to the tumor-adjacent tissues. Pearson chi-square test was used to analyze the relationship between the miR-218 expression and clinical features. The expression of survivin was measured by immunohistochemistry（IHC）, Spearman correlation analy- sis was used to analyze the relationship between miR-218 and survivin, miR-218 in human TE 1 cells were over expressed and the changes of survivin mRNA and protein were measured. RESUITS The relative expression of miR-218 was signif- icantly down-regulated in ESCC tissues compared to the matched tumor-adjacent tissueFs （4. 392±0. 203 vs 9. 301 ± 0. 211, P〈0. 001）. Low expression of miR-218 was significantly associated with large tumor size （〉5 cm, t= 2. 872, P=0. 006） and advanced TNM stage （Ⅲ±Ⅳ, t= 3. 231, P= 0. 003）. The expressed correlation between survivin and miR-218 was significantly negative （P〈0.05）. Both the mRNA and protein expression of survivin in TE-1 cells were down-regulated after transfection （P〈0.05）. CONCLUSION Low expression of miR-218 is correlated to the malignant clinicopathological features in ESCC tissues, and miR 218 may suppress ESCC through down-regulating survivin.

关 键 词：microRNA-218 SURVIVIN 表达 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]