两色金鸡菊乙酸乙酯提取物对STZ诱导的糖尿病SD大鼠的影响  

Effect of ethyl acetate extract from coreopsis tinctoria on diabetic SD rats induced by STZ

作　　者：张雨洁[1] 兰怡 李卉[3] 姚蓝[1] 李琳琳[4] 王健[4] 毛新民[1] 

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830054 [2]新疆维吾尔自治区中医医院干部二科,新疆乌鲁木齐830000 [3]新疆医科大学中心实验室,新疆乌鲁木齐830054 [4]新疆医科大学基础医学院药理教研室,新疆乌鲁木齐830054

出　　处：《中国药理学通报》2015年第10期1439-1443,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金-新疆联合基金重点支持项目(No U1303223)

摘　　要：目的观察两色金鸡菊乙酸乙酯提取物对糖尿病大鼠糖脂代谢及肝脏、肾脏功能的影响。方法高糖高脂喂养联合腹腔注射链脲佐菌素(streptozotocin,STZ)建立2型糖尿病SD大鼠模型,成模大鼠随机分6组(正常组、模型组、两色金鸡菊乙酸乙酯提取物高、中、低3个剂量组0.15、0.3、0.6 g·kg-1、阳性药二甲双胍0.16 g·kg-1组)。正常组和模型组灌胃生理盐水,其余组每天灌胃给药两色金鸡菊提取物,每周测体重及随机血糖,给药4周后处死,收集大鼠血清,检测血脂四项、肝功肾功指标、血清胰岛素及糖化血红蛋白水平。结果两色金鸡菊乙酸乙酯提取物可有效降低糖尿病大鼠的随机血糖和糖化血红蛋白及血清甘油三酯、低密度脂蛋白、血清总蛋白、血肌酐及血尿酸水平,同时能升高糖尿病大鼠血清白蛋白的含量。结论两色金鸡菊乙酸乙酯提取物能降低糖尿病SD大鼠的血糖血脂,保护其肝肾功能。Aim To observe the effects of the ethyl ace-tate extract from Coreopsis tinctoria on glucose and lipid metabolism and liver, kidney function in diabetic rats. Method By high-sugar, high-fat diet combined with intraperitoneal injection of streptozotocin ( streptozoto-cin, STZ ) Type 2 diabetes SD rat model was estab-lished. Model rats were randomly divided into six groups ( control group, model group, three dose groups Coreopsis tinctoria extract:low, middle,high 0. 15 g· kg-1;0. 3 g·kg-1;0. 6 g·kg-1 , positive drug met-formin 0. 16 g · kg-1 group ) . The control group and the model group were given physiological saline and the remaining groups intragastric administration coreofosis tinctoria extrat. Random blood glucose and body weight of rats were measured weekly. After 4 weeks of admin-istration, The rats were killed and rat serum was col-lected to detect serum lipids ( TC/TG/HDL/LDL ) , liver and renal function indicators, serum insulin, and glycated hemoglobin levels. Result Coreopsis tincto-ria ethyl acetate extract effectively reduced the diabetic rats random blood glucose, glycated hemoglobin,serum triglycerides, LDL, total serum protein, serum creati-nine and uric acid levels, and increased serum white protein content in diabetic rats. Conclusion Coreop-sis tinctoria ethyl acetate extract can reduce blood glu-cose and lipid in diabetic SD rats and protect their liver and kidney function.

关 键 词：两色金鸡菊 乙酸乙酯提取物 糖尿病 SD大鼠 灌胃 STZ 二甲双胍 

分 类 号：R-332[医药卫生] R284.1