三七皂苷R1对肺高压大鼠肺动脉平滑肌细胞SOCE的抑制作用  

Inhibition of notoginsenoside R1 on SOCE in pulmonary arterial smooth muscle cells of pulmonary hypertension rats

作　　者：王瑞幸[1] 戴耄 穆云萍[1] 江娇[1] 黄秋虹[1] 吴枝娟[1] 焦海霞[1] 林默君[1] 

机构地区：[1]福建医科大学基础医学院生理学与病理生理学系心血管科学研究室,福建福州350108

出　　处：《中国药理学通报》2015年第10期1463-1468,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31171104;31371165;81400236);福建省自然科学基金资助项目(No 2015J01313)

摘　　要：目的探讨三七皂苷R1(notoginsenoside R1)对慢性低氧(chronic hypoxia,CH)及野百合碱(monocrotaline,MCT)致肺高压(pulmonary hypertension,PH)大鼠肺动脉平滑肌细胞(pulmonary arterial smooth muscle cells,PASMCs)钙池操纵性钙内流(store-operated calcium entry,SOCE)的作用。方法制备CH及MCT致PH大鼠模型,通过Mn2+淬灭Fura-2荧光和Fluo-3荧光检测胞质游离Ca2+浓度(intracellular free calcium concentration,[Ca2+]i)观察三七皂苷R1对CH及MCT致PH大鼠PASMCs SOCE的作用。结果成功制备CH及MCT致PH大鼠模型;在硝苯地平预处理情况下,10μmol·L-1三七皂苷R1可明显降低环匹阿尼酸(cyclopiazonic acid,CPA)诱导CH及MCT致PH大鼠PASMCs Mn2+淬灭幅度、Mn2+最大淬灭率、胞膜Ca2+内流量和静息[Ca2+]i。结论三七皂苷R1对CH及MCT致PH大鼠PASMCs具有抑制SOCE和降低静息[Ca2+]i的作用。Aim To evaluate the effects of notoginsen-oside R1 on store-operated calcium entry ( SOCE ) in pulmonary arterial smooth muscle cells ( PASMCs ) of chronic hypoxia ( CH)-and monocrotaline ( MCT)-in-duced pulmonary hypertension ( PH) rats. Methods Mn2+ quenching of Fura-2 and measurement of intra-cellular free calcium concentration ( [ Ca2+] i ) using fluo-3 were examined in PASMCs of CH-exposed and MCT-treated rats. Results ①CH-exposed and MCT-treated rats exhibited profound PH when examined 3 weeks after hypoxia exposure or MCT injection, respec-tively. ②In the presence of 3 μmol·L-1 nifedipine, 10 μmol · L-1 notoginsenoside R1 significantly re-duced cyclopiazonic acid ( CPA )-induced the percent reduction in Fura-2 fluorescence measured 500 sec af-ter application of Mn2+, the maximal rate of Mn2+quenching, the amplitude of the Ca2+ influx transient and the resting [ Ca2+] i in PASMCs of CH-exposed and MCT-treated rats. Conclusion Notoginsenoside R1 inhibits SOCE and reduces resting [ Ca2+] i in PASMCs of CH-and MCT-induced PH rats.

关 键 词：肺高压 慢性低氧 野百合碱 三七皂苷R1 钙池操纵性钙内流 肺动脉平滑肌细胞 

分 类 号：R-332[医药卫生] R284.1