组织自发荧光在大鼠局灶性脑缺血/再灌注损伤中的应用  

Application of tissue spontaneous fluorescence in draw focal cerebral ischemia reperfusion injury of rats

作　　者：田树红[1] 王日超 邢桂兰[1] 符健[1] 

机构地区：[1]海南医学院海南省药物安全性评价研究中心,海南海口571199

出　　处：《中国药理学通报》2015年第10期1473-1476,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81160408)

摘　　要：目的基于特定的荧光检测技术,探讨组织自发荧光在大鼠局灶性脑缺血及再灌注损伤中的应用。方法本研究通过caliper精诺真IVIS活体成像技术检测大鼠脑组织的自发荧光。通过荧光检测,比较正常大鼠和局灶性脑缺血/再灌注损伤大鼠脑组织对应部位自发荧光强弱的变化,并进行定量分析。结果实验结果表明,局灶性脑缺血/再灌注损伤脑组织的自发荧光发生了明显变化,损伤部位的自发荧光信号明显增强,将荧光信号通过活体成像系统进行定量,与正常脑组织比较差异有统计学意义,P<0.01。结论大鼠脑组织在局灶性脑缺血/再灌注损伤后,脑组织的自发荧光明显增强,为大鼠局灶性脑缺血/再灌注损伤模型的研究及其评价提供新的方法。Aim To discuss the application of tissue spontane-ous fluorescence for draw focal cerebral ischemia reperfusion in-jury in rats based on specific fluorescence detection technology. Methods The change of spontaneous fluorescence WAS COM-PARED between the brains of normal rats and those of rats with draw focal cerebral ischemia-reperfusion injury and an quantita-tive analysis was then made. Result The results showed that spontaneous fluorescence of brain tissue for focal cerebral ische-mia reperfusion injury changed significantly. Spontaneous fluo-rescence signal of injury considerably enhanced. The fluores-cence signal which was quantified by IVIS had significant statisti-cal significance compared with normal brain tissue, P <0. 05. Conclusion Our research shows that spontaneous fluorescence of brain tissue enhances obviously after focal cerebral ischemia-reperfusion injury. Our research provides a method for the re-search and evaluation of focal cerebral ischemia-reperfusion inju-ry model in rats.

关 键 词：大鼠 脑组织 自发荧光 局灶性脑缺血/再灌注 活体成像系统 

分 类 号：R-332[医药卫生] R322.81