易致污染的支原体在固体培养基上的形态特征  

Morphology on solid media for mycoplasmas susceptible to cause contaimination

作　　者：傅元欣[1] 魏然[1] 宣俊文[1] 于佳[1] 王轶峰[1] 姜宝芹[1] 刘晓凡[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2015年第5期17-22,共6页Progress In Microbiology and Immunology

摘　　要：目的：研究不同条件下、不同生长期易致污染的支原体在固体培养基中的形态特征。方法通过改变支原体菌体浓度、培养时间及培养基组成，观察支原体在固体培养基中的形态变化。结果支原体菌体浓度小于103 cfu／mL时，培养至2～3天后可在固体培养基中形成典型的“油煎蛋”状形态，以蔗糖作为碳源培养支原体会导致葡萄糖发酵型支原体（肺炎支原体）不能在固体培养基中形成典型菌落，当使用牛血清代替马血清为支原体提供外源胆固醇时，猪鼻支原体在固体培养基中不能形成典型菌落。结论支原体的不同生长期、菌体浓度、培养基的组成都会使易致污染的支原体在固体培养基上的形态发生变化。Objective To study the morphology on solid media for mycoplasmas susceptible to cause contaimination under different conditions and culture periods.Methods To observe the morphological characteristics of mycoplasmas cultured on solid medium by changing the cell concentration, composition in medium and incubation conditions for tested myco-plasmas.Results After cultured for two or three days, the tested mycoplasma colonies have typical “fried egg” appear-ance on solid media as the cell concentration is less than 103 cfu/mL.Mycoplasma pneumonia can not form a typical colony on solid medium as sucrose used as a carbon source, Mycoplasma hyorhinis can not form a typical“fried egg” appearence by using of bovine serum instead of horse serum as an exogenous cholesterol.Conclusion The morphology of tested mycoplasmas may change on solid medium under different culture time, different cell concentration and different medium composition.

关 键 词：肺炎支原体 口腔支原体 猪鼻支原体 固体培养基 形态 

分 类 号：Q93-33[生物学—微生物学]