三种除蛋白方法对复方乙肝宁多糖及蛋白的影响  

Influence of three methods of removing protein about compound Polysaccharide in Yiganning

作　　者：李静[1] 王定雪[2] 

机构地区：[1]贵阳中医学院实验中心,贵州贵阳550002 [2]贵阳中医学院第一附属医院,贵州贵阳550002

出　　处：《中国民族民间医药》2015年第18期20-22,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:对乙肝宁复方多糖进行三种除蛋白处理,考查除蛋白效果。方法:采用苯酚-硫酸法在490 nm处测定糖的含量,以多糖回收率和蛋白去除率为指标。结果:Sevag法、5%三氯乙酸法、三氯乙酸-正丁醇法的多糖回收率分别为57.41%、88.27%、64.90%,蛋白去除率分别为53.06%、20.23%、28.90%。结论:5%三氯乙酸法对多糖的保留效果最好,Sevag法除蛋白效果最好,本实验为多糖除蛋白的方法提供了实验依据。Objective In order to test effect to three methods to removing protein of compound polysaccharides in Yiganning. Methods The polysaccharide content was determined by phenol - sulfuric acid method comparing colors at 490 nm. With polysaccha- ride recovery rate and protein removal rate as an indicator. Results Sevag method, 5% TCA method , TCA -Butanol method of poly- saccharide recovery rates were 57.41%, 88. 27%, 64. 90%, while protein removal rates were 53.06%, 20. 23%, 28.90%. Con- clusion 5% TCA method on polysaccharide retention effect is better, Sevag method protein removal effect is better. The experiment provides basis for the study of removing protein method.

关 键 词：乙肝宁复方 多糖 苯酚-硫酸法 除蛋白 

分 类 号：R914[医药卫生—药物化学]