不同压力氧气对大鼠潜水减压病的预防作用  

Effect of different pressure oxygen pre-breathe in diving decompression sickness of rats

作　　者：王芳芳[1] 方以群[1] 攸璞[1] 包晓辰[1] 马骏[1] 张师[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中国应用生理学杂志》2015年第5期401-404,共4页Chinese Journal of Applied Physiology

基　　金：海军后勤部科研基金项目(12A106);总后十二五重点科研课题(BWS11J053;BHJ12J003)

摘　　要：目的:研究不同压力氧气对大鼠减压病的预防作用。方法:40只雄性SD大鼠,随机分为减压病组、1 ATA预吸氧组、2 ATA预吸氧组、3 ATA预吸氧组。减压病组置于脱险舱内,以"压缩空气3 min内匀速加压至0.7 MPa,停留60 min后,3 min内匀速减压"方案处理后出舱。预吸氧组分别于进舱前吸不同压力的氧20 min后再按上述方案加减压出舱。出舱后30 min内观测大鼠死亡率、行为学改变;取大鼠肺组织,测其干湿重比、支气管肺泡灌洗液蛋白量以及肿瘤坏死因子(TNF-α)表达含量变化。结果:和减压病组比较,预吸氧对减压病的死亡率及发病率无明显影响。但1 ATA预吸氧组大鼠支气管肺泡灌洗液蛋白量和炎症因子TNF-α值下降明显,肺干湿重比升高明显(P<0.05)。结论:预吸氧对减压病的死亡率及发病率无明显改善,但是常压吸氧可减轻存活大鼠肺泡灌洗液中蛋白渗透、降低肺组织中炎症因子的表达。Objective： To investigate the effect of different pressure oxygen pre-breathing in preventing decompression sickness of rats. Methods： Forty male SD rats were randomly divided into 4 groups： decompression sickness （DCS） group and three oxygen pre-breathing groups with 1 ATA, 2 ATA and 3 ATA pressure respectively. The rats of DCS group were placed in the hyperbaric chamber and the chamber was compressed evenly within 3 minutes to depths of 7 absolute atmosphere（ATA） and held at the designated depth for 60 min, then decom- pressed （3 rain） at constant speed to the surface pressure. After that, the rats were taken out for further detection. While the rats of oxygen pretreatment groups pre-breathed different pressure oxygen for 20 min before entering into chamber. The mortality and behavioral of rats were observed with 30 min post decompression. The dry/wet ratio of the lung, protein levels in the brenchoalveolar lavage fluid （BALF）, and the inflammatory cytokine tumor necrosis factor （TNF-a） expression were also tested. Results： Compared with that of the DCS group, the mortality and morbidity of oxygen pre-breathe groups didn＇ t change obviously. But the total BALF protein level and the inflammatory cytokine TNF-a ex- pression of 1 ATA oxygen pre-breathe group were obviously decreased, while the dry/wet ratio of lung as obviously increased instead（ P 〈 0.05）. Conclusion： Although preoxygenation can＇ t obviously change the mortality and mobidity of rats, normal pressure oxygen pre-breathing can mitigate the protein infiltration in BALF and the expression of inflammatory cytokine in lung tissue.

关 键 词：预吸氧 减压病 炎症因子 大鼠 

分 类 号：R733[医药卫生—肿瘤]