配伍对君药酸枣仁中斯皮诺素和阿魏酸的药动学影响  

Pharmacokinetic effect of combined administration on spinosin and ferulic acid in monarch drug Ziziphi Spinosae Semen kernel

作　　者：高蓉[1] 李珊[1] 陈贤金 王晓锋[1] 王世祥[1] 房敏峰[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西安中学,陕西西安710018

出　　处：《中国中药杂志》2015年第16期3293-3297,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(J1210063);陕西省重点科技创新团队计划项目(2013KCT-24);陕西省"春笋计划"课题研究项目(201481)

摘　　要：研究枣仁安神方中君药酸枣仁不同配伍对其主要成分在大鼠体内的药代动力学影响。将SD大鼠随机分为酸枣仁组、酸枣仁-五味子组、酸枣仁-丹参组、枣仁安神方组,灌胃给药,HPLC检测0~24 h大鼠血浆中斯皮诺素和阿魏酸血药浓度,流动相乙腈-0.03%磷酸水溶液,梯度洗脱,检测波长280 nm,DAS 2.0软件计算药代动力学参数,比较酸枣仁不同配伍后其主要成分的药动学差异。与酸枣仁单味药组比较,酸枣仁-五味子组和酸枣仁-丹参组中斯皮诺素和阿魏酸的达峰浓度（Cmax）和药时曲线下面积（AUC0-t）降低,清除率CL/F增加,而枣仁安神方组中斯皮诺素和阿魏酸Cmax和AUC0-t增加,CL/F降低。与酸枣仁组相比,复方中主要成分代谢减慢,斯皮诺素和阿魏酸的生物利用度显著提高。To study the pharmacokinetic effect of different combined administration with monarch drug Ziziphi Spinosae Semen on its main components in rats. Sprague-Dawley （SD） rats were randomly divided into Ziziphi Spinosae Semen group, Ziziphi Spinosae Semen-Fructus Schisandrae Chinensis group, Ziziphi spinosae Semen-Salviae Miltiorrhize Radix et Rhizoma group and Zaoren Ansheng prescription group. After oral administration, HPLC was eluted with the mobile phase of acetonitrle-0. 03% phosphate acid water in a gradient mode. The detection wavelength was 280 nm. The pharmacokinetic parameters of spinosin and ferulie acid were calculated by DAS 2. 0 software. Compared with Ziziphi Spinosae Semen group, Ziziphi Spinosae Semen-Fructus Sehisandrae Chinensis group, Ziz- iphi Spinosae Semen-Salviae miltiorrhizae Radix et Rhizoma group showed a lower maximum plasma concentration （ Cmax ） and area un- der curve （AUC0-t ） for spinosin and ferulie acid but higher clearance speed （CL/F） ; whereas the Zaoren Ansheng prescription group showed higher maximum plasma concentration （ Cmax ） and area under curve （ AUC0-t ） for spinosin and ferulic acid but lower clearance speed （CL/F）. Compared with Ziziphi Spinosae Semen group, prescription group showed slower metabolism of spinosin and ferulic acid and higher bioavailability.

关 键 词：枣仁安神方 斯皮诺素 阿魏酸 配伍 药动学 

分 类 号：R285.5[医药卫生—中药学]