α-亚麻酸对人未成熟卵母细胞体外成熟的影响  

Effect of alpha- linolenic acid on in vitro maturation of human immature oocytes

作　　者：孙丽君[1] 胡继君[1] 管一春[1] 武文斌[1] 马晓娟[1] 宋娜[1] 齐若凡 

机构地区：[1]郑州大学第三附属医院生殖医学中心,河南郑州450052

出　　处：《中国妇幼保健》2015年第29期5002-5005,共4页Maternal and Child Health Care of China

基　　金：河南省医学科技攻关计划项目〔201403109〕

摘　　要：目的探讨适宜浓度α-亚麻酸（ALA）对卵胞浆内单精子注射（ICSI）周期中人未成熟卵母细胞体外成熟、受精和胚胎发育的影响。方法选取2014年10月-2015年2月在该院接受ICSI治疗的不孕患者150名为研究对象，将其ICSI周期中未成熟卵母细胞随机分为ALA组（A组）、成熟卵泡液组（B组）和空白对照组（C组）。3组细胞在人未成熟卵母细胞体外成熟培养（IVM）培养液中培养24h，观察未成熟卵母细胞体外成熟情况及胚胎发育情况。同时测定卵母细胞培养基中超氧化物歧化酶（SOD）总活力和丙二醛（MDA）含量。结果A组24h成熟率高于B组，B组高于C组，差异均有统计学意义（P〈0．05）；A组和B组卵母细胞ICSI后2PN卵裂率高于C组，差异有统计学意义（P〈0．05）；3组2PN受精率、可利用胚胎率、囊胚形成率和SOD活力两两比较，差异无统计学意义（P〉0．05）；A组MDA含量低于B组和C组，差异均有统计学意义（P〈0．05）。结论ALA通过降低氧化应激水平促进卵母细胞体外成熟，提高胚胎发育潜能。Objective To investigate the effect of appropriate dose of alpha-linolenic acid （ALA） on in vitro maturation （IVM）, fertilization, and embryo development in intracytoplasmic sperm injection （ICSI） cycles. Methods From October 2014 to February 2015, a total of 150 infertile patients who received ICSI treatment were collected as study objects. Immature oocytes in ICS1 cycle were randomly di- vided into ALA group （group A）, mature follicular fluid group （group B）, and control group （group C ） . Cells in the three groups were cultured in IVM medium of human immature oocytes for 24 hours. The oocytes＂ maturation and embryo development were observed. Mean- while, superoxide dismutase （SOD） activity and malondialdehyde （MDA） levels were determined in the three groups. Results The 24- hour mature rate of immature oocytes in group A was significantly higher than that in group B （ P〈0. 05 ）, and the rate in group B was higher than that in group C （P〈0. 05） , and the difference was statistically significant. In group A and group B, the cleavage rates of 2PN in oocytes after ICSI were higher than that in group C with statistical significance （P〈0.05） . In the three groups, rates of 2PN fertilization, available embryos and blastocyst formation, and SOD activity between either two groups revealed no statistical difference （P〉0. 05 ） . The level of MDA in group A was lower than those in group B and group C with significant difference （P〈0.05） . Conclusion ALA can promote IVM and increase embryo development potential in oocytes by reducing oxidative stress level.

关 键 词：卵母细胞体外成熟培养 Α-亚麻酸 氧化应激 超氧化物歧化酶 丙二醛 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]