余甘子精粉的体外抗氧化活性分析  

Analysis on Antioxidant Activities in vitro of Refined Powder of Phyllanthus emblica L.

作　　者：李娴[1,2] 甘瑾[1,2] 张雯雯[1,2] 侯彬[1,2] 张弘[1,2] 冯颖[1,2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所 [2]国家林业局资源昆虫培育与利用实验室,云南昆明650224

出　　处：《林业科学研究》2015年第5期753-757,共5页Forest Research

基　　金："十二五"国家科技支撑计划项目子课题(2012BAD36B03-03)

摘　　要：余甘子（PhyllanthusemblicaL．）是大戟科（Eu．phorbiaceae）叶下珠属（Phyllanthus）植物，其果鲜食酸而微涩，回味甘甜，故名余甘，又名滇橄榄、油柑子、庵摩勒等。全世界约有17个国家的传统药物体系中使用余甘子，在我国的中药、藏药等均有应用，作为一味重要的传统民族药，已被载人多版《中国药典》。The antioxidant activities in vitro of refined powder of PhyUanthus emblica L. were detected by methods based on chemical measurement and cell modeling. The results of anti-peroxidation ability and iron reduction ability trials showed that the refined powder of P. emblica L. had strong ability of anti-peroxidation, and it increased with the increasing of concentration with a clear dose-response relationship. When the concentration reached 800 μg · mL^-1, the ability of anti-peroxidation was almost equal to the positive control VC with the same concentration. Meanwhile, it also had iron reduction ability that accompanied by significant dose-response relationship. By in vitro cell culture method, H2O2 was used to induce SH-SY5Y cells damages to establish the cell model of oxidative dam- age. The author detected the effect of different concentrations of powder on cell survival rate for the model cells by MTT assay. The results indicated that refined powder could show better protective effect to oxidative damaged SH- SY5Y cells in the range of 600 -1 200 μg · mL^-l. When the concentration reached 1 200 μg · mL^-l, the cell survival rate was the highest and refined powder exhibited the best antioxidant activity. Both methods proved the strong antioxidant activities of refined powder of P. emblica L. in vitro.

关 键 词：余甘子精粉 体外抗氧化 FRAP法 SH-SY5Y细胞 H2O2损伤 

分 类 号：S667.9[农业科学—果树学]