北极海洋沉积物高抗氧化活性菌株的筛选及其多样性分析  

Screening and Diversity Analysis of the Bacteria With High Antioxidant Activity Isolated From Marine Sediments of Arctic

作　　者：王桢[1,2] 李阳[1,2] 车帅[1,2] 林学政[1,2] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]国家海洋局海洋生物活性物质重点实验室,山东青岛266061

出　　处：《海洋科学进展》2015年第1期63-70,共8页Advances in Marine Science

基　　金：南北极环境综合考察与评估专项--2013年度北极海域海洋生物和生态考察(CHINARE2013-03-05)

摘　　要：利用过氧化氢耐受性实验对北极海洋沉积物中具有高抗氧化活性的菌株进行初筛,通过其对DPPH(1,1-二苯基-2-三硝基苯肼)自由基、羟自由基和超氧阴离子清除能力的测定进一步验证其抗氧化能力;并对其进行了分子鉴定与系统发育分析。根据过氧化氢耐受性实验,从145株供测菌株中筛选出25株对过氧化氢具有较强耐受性的抗氧化活性菌株,占总供测菌株的17.2%;其对DPPH自由基、羟自由基和超氧阴离子均具有较强的清除能力,可分别高达84.15%,88.61%和45.77%。基于16SrRNA基因的分子鉴定与系统发育分析表明,筛选出的25株活性菌株多样性较高,分别属于细菌域的5个纲,15个属,20个种;其中以γ-Proteobactria纲的菌株数量最多。北极海域的海洋沉积物中存在着丰富的具抗氧化活性的菌株资源,将为了解极地微生物的抗氧胁迫机制及极地来源的新型生物活性物质的研发打下一定的基础。The bacteria isolated from Arctic marine sediments with high antioxidant activity were deter- mined by their growth resistance ability to hydrogen peroxide, and their scavenging capacity for DPPH （2, 2-diphenyl-l-picrylhydrazyl）, hydroxyl radical and superoxide anion were also examined. Molecular identi- fication and phylogenetic analysis were carried out on the basis of 16S rRNA gene. Twenty-five antioxidant strains were isolated from 145 strains tested by their growth resistance ability to hydrogen peroxide in vitro, accounting for 17.2% of total strains tested. The results of scavenging test for DPPH free radical, hydroxyl radical and superoxide anion showed that the scavenging ratio were up to 84.82%, 88.89 % and 45. 73%, respectively, indicating these strains had high antioxidant ability. Molecular identification and phylogenetic analysis revealed that these isolates belonged to five classes of the bacteria domain, 15 gene- ra, and 20 species in total, and γ-Proteobactria was dominant. A high diversity of antioxidant bacteria was existing in marine sediment of Arctic in term of both species number and phylogenetic composition, which deserve further study of adaptation mechanisms under oxidative stress and exploitation of novel bioactive substances.

关 键 词：北极 海洋沉积物 抗氧化活性 系统发育分析 

分 类 号：Q751[生物学—分子生物学]