高产铁载体假单胞菌的筛选及其对铁氧化物的利用  

Screening of Pseudomonas Strains Producing High-yield Siderophore and Its Utilization of Iron Hydroxides

作　　者：朱慧明[1] 张彦 杨洪江[1] 

机构地区：[1]天津科技大学生物工程学院工业微生物教育部重点实验室天津市工业微生物重点实验室,天津300457 [2]天津双联科鑫生物科技有限公司,天津300403

出　　处：《生物技术通报》2015年第9期177-182,共6页Biotechnology Bulletin

基　　金：由天津市科技支撑计划重点项目(13ZCZDNC00600)

摘　　要：筛选高产铁载体的微生物,研究铁载体的抑菌作用和对不溶性未定型铁氧化物(poorly crystalline iron hydroxides,PCIH)的利用。CAS法筛选高产铁载体菌株,采用琼脂扩散法和生长抑制测定铁载体的抑菌作用,利用16S r RNA基因序列比对鉴定分离菌株,并根据分离菌株的生长情况,确定铁载体对不溶性PCIH的利用。从土壤样品中共筛选到172株产铁载体的菌株,高产铁载体菌株13株,其中仅有菌株Z158的发酵液对金黄色葡萄球菌、藤黄微球菌、普通变形杆菌和副溶血性弧菌具有抑菌作用,抑菌率分别为51.3%、50.2%、37.1%和28.0%。比对该菌株的16S r RNA基因序列,确定Z158属于Pseudomonas aeruginosa。当不溶性的PCIH作为唯一可利用的铁源时,菌株Z158培养24 h的生物量比无铁条件下提高了46.1%。铜绿假单胞菌Z158分泌的铁载体能够抑制病原菌的生长,同时还能获取不溶性未定型铁氧化物PCIH中的铁元素。The aim of this study is to isolate the microorganisms producing high-yield siderophore, investigate antimicrobial activity of siderophore against pathogenic bacteria, and study the effect of siderophore on the utilization of insoluble poorly crystalline iron hydroxides （PICH）. CAS method was used for isolating microorganisms producing high-yield siderophore. Agar diffusion method and growth inhibition assay were used to test antimicrobial activities of siderophore. Sequence alignment of 16S rRNA gene was used for strain identification. Bacterial growth was measured to investigate the utilization of insoluble PCIH by siderophore. Totally, 172 siderophore-producing strains were isolated from soil samples, including 13 potentially strong siderophore producers（PSSP）. The analysis of antimicrobial activities of siderophore showed that only the supernatant of Z158 culture had a pronounced inhibiting effect on the growth of Staphylococcus aureus, Micrococcus luteus, Proteus vulgaris, and Vibrio parahemolyticus, and the inhibition efficiency was 51.3%, 50.2%, 37.1%, and 28.0%, respectively. Homology analysis of 16S rRNA gene sequence identified strain Z158 as Pseudomonas aeruginosa. Moreover, with insoluble PICH as sole iron source, the biomass of Z158 increased by 46.1%at 24 h after incubation. In conclusion, the siderophore secreted by P. aeruginosa Z158 could inhibit the growth of pathogenic bacteria and acquire iron element from insoluble PCIH.

关 键 词：铁载体 铜绿假单胞菌 抑菌 未定型铁氧化物 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]