皮肤鳞状细胞癌组织中SFRP1基因启动子甲基化研究  

Methylation of SFRP1 gene promoter in cutaneous squamous cell carcinoma tissue

作　　者：梁俊琴[1,2] 伊力努尔·哈力甫[2] 邹云敏[2] 罗东[2] 袁虎[2] 普雄明[2] 

机构地区：[1]新疆医科大学,乌鲁木齐830002 [2]新疆维吾尔自治区人民医院皮肤性病科

出　　处：《中华皮肤科杂志》2015年第10期723-726,共4页Chinese Journal of Dermatology

基　　金：新疆维吾尔自治区自然科学基金（2015211C911）

摘　　要：目的探讨SFRP1与皮肤鳞状细胞癌（鳞癌）临床病理的关系及其在鳞癌发生发展中的可能作用机制。方法用MassARRAY质谱仪EpiTYPER甲基化方法检测鳞癌组织，癌旁组织和正常皮肤组织各40例样本中SFRP1基因启动子甲基化状态。结果共完成SFRP1基因启动子2个片段17个CpG位点1951个CpG单位（1951／2255，86．52％）的甲基化评估，鳞癌与癌旁组织相比、癌旁组织与正常组织相比分别检测出10个CpG位点（10／17，58．82％），5个CpG位点（5／17，29．41％）甲基化率差异有统计学意义（P〈0．05）。检测到SFRP1基因启动子3个位点在不同组织病理级别差异有统计学意义（P〈0．05），其甲基化率鳞癌Ⅲ级〉鳞癌Ⅱ级〉鳞癌Ⅰ级。结论SFRP1在皮肤鳞痛中旱现高甲某化率．可能参与了鳞癌的发生发展过程．Objective To investigate the relationship between SFRP1 gene and elinicopathologic features of cutaneous squamous cell carcinoma （CSCC）, and to explore the possible mechanism of action of SFRP1 in the occurrence and development of CSCC. Methods CSCC and paracarcinomatous tissue specimens were obtained from 40 patients with CSCC, and normal skin tissue specimens from 40 healthy human controls. The EpiTYPER assay was conducted to evaluate the methylation status of SFRP1 gene promoter in all the specimens with a MassARRAY mass spectrometer. Results Totally, the methylation status of 1951 （86.52%, 1951/2255） CpG motifs were evaluated in 17 CpG loci in 2 fragments of the SFRP1 gene promoter. The methylation rate significantly differed in 10 （ 10/17, 58.82%） CpG loci between the CSCC and paracarcinomatous tissue specimens, and in 5 （5/17, 29.41%） CpG loci between the paracarcinomatous and normal tissue specimens （all P 〈 0.05）. Furthermore, significant differences were observed in the methylation rates of three CpG loci （CpG 1_5, CpG 1_7, CpG 2_8） in the SFRP1 gene promoter between tissue specimens from different pathological grades of CSCC （P 〈 0.05 ）, and their methylation rates sequentially decreased from grade Ⅲto grade Ⅱand Ⅰ. Conclusion The frequency of methylation is high in the SFRP1 gene promoter in patients with CSCC, and the SFRP1 gene may participate in the occurrence and development of CSCC.

关 键 词：癌 鳞状细胞 DNA甲基化 信号传导 SFRP1 基因启动子 

分 类 号：R739.5[医药卫生—肿瘤]