TGF-β3和DPSCs修复兔面神经损伤的组织学观察  

Histological observation on the effects of TGF-β3 and dental pulp stem cells in repair of rabbit facial nerve injury

作　　者：庄友梅[1] 木合塔尔·霍加[1] 马蓉[1] 张晓莉[1] 许辉[2] 

机构地区：[1]新疆维吾尔自治区人民医院口腔科,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院颌面外科

出　　处：《国际生物医学工程杂志》2015年第4期222-224,229,I0007,共5页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（30960423）

摘　　要：目的探讨联合应用质量浓度为100ng／pJ的转化生长因子β3（TGF-β3）和牙髓干细胞（DPSCs）共同修复兔面神经横断性损伤的可行性。方法选用健康成年新西兰大白兔36只，随机分为实验组（DPSCs＋TGF-β3组）、对照1组（TGF-β3组）和对照2组（PBS组），每组各12只。在兔左面颊部手术建立面神经上颊支横断伤模型，实验组向再生室内加入质量浓度为100ng／txl的TGF-β3液和浓度为lxl0S／L的DPSCs悬液0．1ml，TGF-β3组加入等体积的质量浓度为100ng／μl的TGF—β3液，PBS组加入等体积的PBS，分别于术后第1、4、12周处死动物制备标本，评价面神经再生及功能恢复情况。结果36只模型全部进入结果分析，与对照组相比，实验组再生神经恢复均优于2个对照组，对照1组优于对照2组，其差异均具有统计学意义（P〈0．05），且各组恢复效果随时间延长越来越好。结论联合应用TGF-β3与DPSCs可有效促进兔面神经再生，且2者联合应用比单独应用TGF-β3修复效果好，TGF-β3组的效果要优于PBS组。Objective To investigate the feasibility of combing the application of transforming growth factorq33 （TGF-β3） with concentration of 100 ng/μl and dental pulp stem cells （DPSCs） for recovering rabbit facial nerve transverse trauma. Methods Thirty-six healthy adult Zelanian rabbits of clean-grade were selected and randomly divided into group DPSCs＋TGF-β3（experimental group）, group TGF-β3 （control group 1） and group PBS （control group 2） with 12 rabbits in each group. The operations for all three groups were applied at rabbit＇s left cheek. A model of traumatic transection was set on upper buccal branch, then 100 ng/p,1 TGF--β3 solution and 0.1 ml of 1 ×10^8/L DPSCs suspension were added into regeneration chamber for the experimental group, while the same amount of 100 ng/μl TGF-β3 solution was added for group TGF-β3 and the same amount of PBS for group PBS. The recovery of facial nerve regeneration with the prepared animal＇s specimen was evaluated in the 1st, 4th and 12th week after the operation on sacrificed rabbits. Results The effects on nerve regeneration recovery for the experimental group was superior to that of control groups 1 and 2 with all the 36 models included in the result analysis, and that of control group 1 was superior to that of control group 2, which was getting better with the extension of time. Conclusions The combined application of TGF-β3 and DPSCs can effectively promote the facial nerve regeneration, which is better than that of single application of TGF-β3. Meanwhile, the effect with TGF-β3 application is better than that with application of PBS.

关 键 词：TGF-Β3 面神经损伤 牙髓干细胞 

分 类 号：R745.12[医药卫生—神经病学与精神病学] Q813[医药卫生—临床医学]