白首乌苷B抗结肠癌作用的研究  

Evaluation of CGB for Colon Cancer Treatment

作　　者：陈蒋丽 张玲[1] 李艳芳[1] 张如松[1] 杨苏蓓[2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江省中药研究所有限公司,浙江杭州310023

出　　处：《中华中医药学刊》2015年第10期2370-2374,I0009-I0010,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家卫生部科学研究基金项目(WKJ2006-2-020)

摘　　要：目的:评价白首乌苷B(简称CGB)体内外抗结肠癌作用,并探讨其作用机制。方法:采用MTT法检测CT-26、HT-29、SW-620和HCT-116四种结肠癌细胞在CGB处理24、48、72 h后的细胞活性;采用流式细胞仪检测PI单染和Annexin V-FITC/PI双染后的细胞周期及细胞凋亡率;采用Western Blot方法测定细胞内周期蛋白的水平;采用CT-26小鼠和HT-29裸鼠移植性肿瘤模型,评价CGB的体内抗肿瘤作用。结果:CGB体外对四种结肠癌细胞有明显的增殖抑制作用,并呈剂量和时间依赖性;CGB能增加结肠癌细胞G0/G1期的比例,同时降低S期和G2/M期的比例,使细胞阻滞于G1期而无法进入S期;CGB能增加晚期凋亡细胞比例,但对早期凋亡和坏死细胞无明显影响;CGB能下调结肠癌细胞中周期蛋白CDK6、cyclin D1和CDK4的表达,且具有明显的量-效关系;CGB对移植性CT-26和HT-29肿瘤的生长具有明显的抑制作用。结论:CGB具有良好的抗结肠癌作用,其作用机制与诱导细胞凋亡、下调周期蛋白的表达、阻滞细胞周期、促使肿瘤组织坏死有关。Objective ： To evaluate the inhibitory effect of CGB on colon cancer in vivo and vitro and discuss the inhibi- tory mechanism. Methods：MTT was used to detect the activities of colon cancer cells CT -26, HT -29, SW -620 and HCT- 116 after treated with CGB, The flow cytometry was used to analyze the cell cycle phrase and apoptosis was meas- ured by Annexin V - FITC/PI double labeled assay and Western Blot was used to analyze the changes of cell cycle protein. And thus to discuss the inhibitory mechanism of CGB. The anti - tumor effect in vivo was determined on the transplant tumor model of CT - 26 and HT - 29 mice. Results ： The experiment showed that different concentrations of CGB showed significant effects on colon cancer cells in vitro. CGB can increase the proportions of GO/G1 phase of colon cancer cells and reduce the proportions of S phase and G2/M phase at the same time, causing cells arrested in G1 phase and can＇t enter S phase. CGB can increase the proportions of late apoptosis,but had no obvious effect on early apoptosis and necrosis cells. CGB can control the expressions of CDK6 ,cyclinD1 and CDK4 and has a significant amount- effect relationship. CGB also has an obvious inhibitory effect on transplant tumor model of CT -26 and HT -29 mice. Conchtsiou： CGB has a good effect on colon cancer treatment and its mechanism of action related to inducing apoptosis, controlling the expression of cycle protein, arresting cell cycle and speeding up necrosis of tumor tissue.

关 键 词：白首乌 白首乌苷B 结肠癌 增殖 凋亡 细胞周期 

分 类 号：R735.35[医药卫生—肿瘤]