冻融产氨棒杆菌细胞转化法制备三磷酸腺苷  

Preparation of ATP by permeable Corynebacterium ammoniagenes cells with frozen-thawed processing

作　　者：丰文飞 欧志敏[1] 沈绍传[2] 贠军贤[2] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032 [2]浙江工业大学化工学院绿色化学合成技术国家重点实验室培育基地,浙江杭州310032

出　　处：《生物加工过程》2015年第5期1-7,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21036005);科技部中欧政府间国际合作专项(1017);浙江省自然科学基金(LZ14B060001)

摘　　要：微生物发酵和酶转化法是工业上制备三磷酸腺苷(ATP)的有效途径。以腺嘌呤为关键底物,用冻融通透化处理的产氨棒杆菌细胞转化制备ATP,用高效液相色谱(HPLC)法检测ATP含量,考察各种转化条件对ATP产率的影响,确定最优转化条件:菌体用量40 g/L,底物6 g/L,葡萄糖60 g/L,Mg SO415 mmol/L,KH2PO4120 mmol/L,反应液p H 7.4,反应温度35℃。在最优转化条件下,ATP产率达到85.00%,比优化前提高了58%,细胞用量大幅度降低,优化条件稳定可行。In industrial process, ATP can be produced by fermentation with microbial strains or biotransformation with enzymes. In this work,permeable Corynebacterium ammoniagenes cells with frozen-thawed processing were used to synthesize ATP using adenine as the primary substrate. The preparation conditions were studied experimentally and ATP concentration in the culture broth was detected by high performance liquid chromatography （HPLC）. The optimum condition was observed,i.e.,wet cells 40 g/L,adenine 6 g/L,glucose 60 g/L,MgSO4 15 mmol/L,KH2PO4 120 mmol/L,pH 7-4 and the reaction temperature at 35 ℃.As a result,adenine was biotransformed successfully into ATP with the yield about 85-00%,which was 58% higher than that before the optimization. The amount of cells needed in the present process was much less than those without frozen-thawed processing. The optimum conditions obtained were stable and feasible.

关 键 词：产氨棒杆菌 三磷酸腺苷 生物转化 腺嘌呤 

分 类 号：TQ92[轻工技术与工程—发酵工程]