产脑苷脂鸡枞菌Termitomyces clypeatus CTM-1的分子生物学鉴定及生长特性研究  

Identification and growth characterization of a cerebroside producing Termitomyces clypeatus CTM-1

作　　者：花卫俊 缪冶炼[1] 陈介余 尤业兵 孙唱[1] 袁丽红[3] 许琳[3] 

机构地区：[1]南京工业大学食品与轻工学院,江苏南京211800 [2]日本秋田县立大学生物资源学部 [3]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《生物加工过程》2015年第5期67-73,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2011CBA00807)

摘　　要：脑苷脂A和B具有显著的镇痛作用和脑神经保护作用。鸡枞菌(Termitomyces clypeatus)子实体含有脑苷脂A和B,但是野生鸡枞菌子实体资源稀少。笔者在前期研究中以野生鸡枞菌子实体为原料,通过组织分离、菌种纯化及筛选,培育出菌株CTM-1。本研究中,采用分子生物学方法鉴定了菌株CTM-1与鸡枞菌的菌种同源性,测定了该菌株平板培养、液体培养中菌丝体的生长速率和脑苷脂含量。实验结果表明:菌株CTM-1及其出发野生子实体与Termitomyces clypeatus的同源性为99%。在PDAY平板培养中,菌落直径的增长速率在27 d内基本稳定在1.5mm/d。比菌落直径增长速率在1 d时为0.21 d-1,然后逐渐下降。在DPt液体培养中,9 d以后为菌丝体生长的稳定期,此时的生物量为3.57 g/L。比菌丝体生长速率在1 d时为2.00 d-1,然后逐渐下降。菌丝体的脑苷脂A、B含量分别为0.152%、0.066%。由此可见,鸡枞菌CTM-1的大规模培养可以成为脑苷脂A、B原料生产的有效途径。Cerebrosides A and B have significant effects of analgesia and brain protection. Fruiting bodies of Termitomyces contains cerebrosides A and B, but wild Termitomyces resources are scarce. In author′s previous work, a strain CTM？1 was developed by means of tissue isolation, strain purification and screening,using the fresh fruiting bodies of wild Termitomyces as raw material. In the present study, the sequence homology of CTM？1 and Termitomyces was identified with a molecular？biological method. The mycelium growth rate in plate and liquid culture,and the cerebroside content in mycelia were investigated for the strain CTM？1. It was shown that the stain CTM？1 and its parental fruiting bodies had a sequence homology up to 99% with Termitomyces clypeatus. In PDAY plate culture,the colony？diameter increasingnbsp;rate was constant at 1？5 mm/d in the period between and 27 d. The specific colony？diameter increasing rate was 0？21 d-1 at 1 d,and decreased gradually after then.In DPt liquid culture,the growth of mycelia entered the stable phase at 9 d,and the biomass was 3？57 g/L. The specific mycelium growth rate was 2？00 d-1 at 1 d,and decreased gradually after then. The content of cerebrosides A and B in mycelia was 0？152% and 0？066%, respectively. Consequently, the large？scale culture of T. clypeatus CTM？1 can be considered as an effective way for the production of raw material containing cerebrosides A and B.

关 键 词：鸡枞菌 菌丝体 脑苷脂 平板培养 液体培养 

分 类 号：Q939.99[生物学—微生物学]