BMSCs来源的外泌体对小鼠乳腺癌细胞4T1增殖、侵袭的影响及机制探讨  

Influence of BMSCs-derived exosome on proliferation and invasion of mouse breast cancer cells 4T1 and the mechanism

作　　者：王丹丹[1] 陈建中[1] 亢春彦[1] 

机构地区：[1]郑州大学第二附属医院,郑州450014

出　　处：《山东医药》2015年第35期12-15,共4页Shandong Medical Journal

摘　　要：目的观察骨髓间充质干细胞(BMSCs)来源的外泌体(exosome)对小鼠乳腺癌细胞4T1增殖、侵袭的影响,并探讨其可能机制。方法将小鼠乳腺癌细胞4T1随机分为3组,4T1+vehicle组仅加入400μL无血清培养基,4T1+exosome组加入400μL由无血清培养基配置的exosome,4T1+exosome+磷脂酰肌醇3激酶(PI3K)/Akt信号通路阻断剂(Y294002)组加入400μL终浓度为5μmol/m L Y294002及400μg/m L exosome的培养基。分别采用MTT法、细胞划痕实验、Western blotting法检测各组细胞增殖、迁移和侵袭能力以及PI3K/Akt信号通路相关蛋白。结果 4T1+exosome组、4T1+vehicle组、4T1+exosome+Y294002组细胞增殖抑制率分别为0.713%±0.050%、0.401%±0.030%、0.459%±0.800%,4T1+exosome组分别与4T1+vehicle组、4T1+exosome+Y294002组比较,P均<0.05。4T1+exosome组、4T1+vehicle组、4T1+exosome+Y294002组细胞迁移距离分别为(388.0±36.1)、(295.0±34.2)、(275.0±63.5)μm,4T1+exosome组分别与4T1+vehicle组、4T1+exosome+Y294002组比较,P均<0.05。4T1+exosome组p-AKT、β-catenin OD值分别为0.30±0.11、0.30±0.08,4T1+vehicle组分别为1.10±0.41、0.70±0.08,4T1+exosome+Y294002组分别为0.40±0.13、0.30±0.07,4T1+exosome组分别与4T1+vehicle组、4T1+exosome+Y294002组比较,P均<0.05。结论 BMSCs来源的exosome能够增加小鼠乳腺癌细胞4T1的增殖、迁移及侵袭能力,其机制可能与上调PI3K/Akt信号通路有关。Objective To observe the influence of bone marrow mesenchymal stem cells （ BMSCs）-derived exosome on the proliferation and invasion of mouse breast cancer cells 4T1 and to investigate the mechanism.Methods The mouse breast cancer cells 4T1 were randomly divided into three groups：4T1＋vehicle group, 4T1＋exosome group and 4T1＋exosome＋Y294002 （an inhibitor of PI3K/Akt signaling pathway） group.4T1＋vehicle group was added with 400μL se-rum-free medium, 4T1＋exosome group was added with 400 μL serum-free medium containing 400 μg/mL exosome and 4T1＋exosome＋Y294002 group was added with 400 μL serum-free medium containing 400 μg/mL exosome and 5 μg/mL Y294002.We used the MTT, cell scratch test and Western blotting to examine the effect of BMSCs-derived exosome on the proliferation, migration and invasion abilities as well as the PI3K/Akt signaling pathway of 4T1 cells.Results The in-hibition rates of cell proliferation of 4T1＋vehicle group, 4T1＋exosome group and 4T1＋exosome＋Y294002 group were 0.713%±0.05%, 0.401%±0.03%and 0.459%±0.80%, respectively.Significant difference was found between 4T1＋exosome group and 4T1＋vehicle group, 4T1＋exosome＋Y294002 group （all P〈0.05）.The cell migration distance of 4T1＋vehicle group, 4T1＋exosome group and 4T1＋exosome＋Y294002 group was （388.0 ±36.1）, （295.0 ± 34.2） and （275.0 ±63.5） μm, respectively.Significant difference was found between 4T1＋exosome group and 4T1＋vehicle group, 4T1＋exosome＋Y294002 group （all P〈0.05）.The OD values of p-AKT and β-catenin of 4T1＋exo-some group were 0.3 ±0.11 and 0.3 ±0.08, they were 1.1 ±0.41 and 0.7 ±0.08 in 4T1＋vehicle group, 0.4 ±0.13 and 0.3 ±0.07 in the 4T1＋exosome＋Y294002 group.Significant difference was found between 4T1＋exosome group＆amp;nbsp;and 4T1＋vehicle group, 4T1＋exosome＋Y294002 group （all P〈0.05）.Conclusion BMSCs-derived exosome can promote the proliferation, migration and invasion abilities of mouse breast cancer cells 4

关 键 词：骨髓间充质干细胞 外泌体 乳腺肿瘤 乳腺癌 PI3K/AKT信号通路 

分 类 号：R739.7[医药卫生—肿瘤]