Expression of p53, p21^(CIP1/WAF1) and eIF4E in the adjacent tissues of oral squamous cell carcinoma:establishing the molecular boundary and a cancer progression model  

作　　者：Yi Li Bo Li Bo Xu Bo Han Hui Xia Qian-Ming Chen Long-Jiang Li 

机构地区：[1]State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University [2]Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University [3]Department of Stomatology, Central Hospital of Panzhihua City

出　　处：《International Journal of Oral Science》2015年第3期161-168,共8页国际口腔科学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 81001209 and 81172578);the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (Grant No. 81321002)

摘　　要：The present study evaluated the expression of key molecules and the status of DNA in both oral squamous cell carcinoma（OSCC） and adjacent tissues to establish a molecular surgical boundary and provide a cancer progression model. Biopsy samples from 50 OSCC patients were divided into T（cancer）, P1（0–0.5 cm）, P2（0.5–1 cm）, P3（1–1.5 cm） and P4（1.5–2 cm） groups based on the distances from the visible boundary of the primary focus. Twenty samples of normal mucosa were used as controls. We used immunohistochemical staining and flow cytometry to evaluate p53, p21CIP1/WAF1, e IF4 E and Ki-67 expression and to determine DNA status, respectively. Sub-mucosal invasion was present in the P1 and P2 groups as determined by haematoxylin and eosin staining.Mutant p53 expression decreased gradually from cancerous to normal mucosae, whereas p21CIP1/WAF1 expression displayed an opposite trend. e IF4 E expression decreased from cancerous to normal mucosae. Ki-67 expression, the heteroploidy ratio, S-phase fraction and proliferative index decreased gradually with the distance from the tumour centre. Based on these results, we suggest that the resection boundary in OSCC surgery should be beyond 2 cm from the tumour. Additionally, the adjacent tissues of the primary focus could be used as a model for assessing cancer progression.The present study evaluated the expression of key molecules and the status of DNA in both oral squamous cell carcinoma（OSCC） and adjacent tissues to establish a molecular surgical boundary and provide a cancer progression model. Biopsy samples from 50 OSCC patients were divided into T（cancer）, P1（0–0.5 cm）, P2（0.5–1 cm）, P3（1–1.5 cm） and P4（1.5–2 cm） groups based on the distances from the visible boundary of the primary focus. Twenty samples of normal mucosa were used as controls. We used immunohistochemical staining and flow cytometry to evaluate p53, p21CIP1/WAF1, e IF4 E and Ki-67 expression and to determine DNA status, respectively. Sub-mucosal invasion was present in the P1 and P2 groups as determined by haematoxylin and eosin staining.Mutant p53 expression decreased gradually from cancerous to normal mucosae, whereas p21CIP1/WAF1 expression displayed an opposite trend. e IF4 E expression decreased from cancerous to normal mucosae. Ki-67 expression, the heteroploidy ratio, S-phase fraction and proliferative index decreased gradually with the distance from the tumour centre. Based on these results, we suggest that the resection boundary in OSCC surgery should be beyond 2 cm from the tumour. Additionally, the adjacent tissues of the primary focus could be used as a model for assessing cancer progression.

关 键 词：e IF4E molecular boundary oral squamous cell carcinoma P21 P53 

分 类 号：R739.8[医药卫生—肿瘤]