茉莉酸甲酯对锦绣杜鹃黄酮的累积及相关酶活性的影响  

Effects of MeJA on flavonoids content and enzyme activities in Rhododendron pulchrum

作　　者：张梅[1] 林棋[1] 娄本勇[1] 潘大仁 周以飞 

机构地区：[1]闽江学院化学与化学工程系,福建福州350108 [2]福建省高校农业生物技术重点实验室,福建福州350002

出　　处：《甘肃农业大学学报》2015年第4期165-169,共5页Journal of Gansu Agricultural University

基　　金：福建省中青年教师教育科研项目(JA14252);国家级大学生创新训练计划项目(201410395016);闽江学院科技项目(YKY12008);闽江学院教学研究与改革项目(MJUB2013025)

摘　　要：以不同质量浓度的茉莉酸甲酯(MeJA)处理锦绣杜鹃植株,分析MeJA对杜鹃叶片黄酮类化合物含量与苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)等主要防御酶活性的变化.结果表明:150μg/mL的MeJA对诱导杜鹃黄酮类化合物的累积和PAL、POD酶的活性影响最为显著.其中,总黄酮含量最大值是对照处理的2.33倍,PAL活性是对照处理的7倍,POD活性是对照处理的2.54倍.而对PPO酶活性影响最显著的是100μg/mL的MeJA处理,PPO活性最大值比对照处理提高了52.51%.研究发现一定质量浓度的茉莉酸甲酯处理杜鹃叶片可明显诱导黄酮类化合物的累积和PAL、PPO、POD等防御酶活性的提高.Effect of methyl jasmonate （MeJA） treatments at different concentration were studied on fla- vonoids content and activity of phenlanine ammonialyase （PAL）,polyphenoloxidase （PPO） and peroxidase （POD） in ＇Rhododendron pulchrum＇ leaves, he results showed that MeJA at 150 μg/mL significantly en- hanced flavonoids content and activity of PAL and POD. The flavonoids content was 2. 33 times,PAL activ- ity was 7 times than that of the control after 8 d of treatment. After the fourth day,POD activity was 2. 54 times than that of the control after 4 d of treatment. MeJA at 100 μg/mL had significantly effect on PPO activity. PPO activity increased by 52. 51% after 8 d of treatment compared with the control. It is suggested that MeJA could induce the flavonoids content and PAL, PPO, POD activities in R. pulchrum leaves.

关 键 词：茉莉酸甲酯 锦绣杜鹃 黄酮类化合物 酶活性 

分 类 号：Q946.8[生物学—植物学]