液相制备油茶叶提取物组分及其结构鉴定  

Compounds and structure identification in extracts from Camellia oleifera leaves by HPLC preparation

作　　者：曹清明[1,2] 钟海雁[1,2] 邬靖宇 包莉圆 孙亚娟[2] 

机构地区：[1]中南林业科技大学经济林培育与保护省部共建教育部重点实验室,湖南长沙410004 [2]中南林业科技大学食品科学与工程学院,湖南长沙410004

出　　处：《经济林研究》2015年第3期68-74,共7页Non-wood Forest Research

基　　金：国家自科科学基金项目"高抗氧活性油茶生物酚组分的结构表征及其作用机理研究"(31070612/C161103)

摘　　要：为了综合开发利用油茶资源,对油茶叶提取物的组分进行了制备和鉴定。将经过大孔树脂和柱分离纯化收集的组分II、III和VIII等油茶叶提取物3个组分进行了HPLC制备,得到了8个纯度较高的化合物,通过薄层显色、高清晰质谱和NMR,鉴定了其中4个化合物为:二氢白藜芦醇(1)、槲皮素(3)、槲皮素-3-鼠李糖苷(6)、1-(3′,5′-二甲氧基)苯基2-[4″-O-β-D-吡喃葡萄糖基(6→1)-O-α-L-吡喃鼠李糖基]苯乙烷(8),其中槲皮素和槲皮素-3-鼠李糖苷是油茶中最常见的2个黄酮类物质,1-(3′,5′-二甲氧基)苯基2-[4′′-O-β-D-吡喃葡萄糖基(6→1)-O-α-L-吡喃鼠李糖基]苯乙烷为第2次报道,但提取率远高于首次报道,二氢白藜芦醇在山茶属植物中属首次报道。In order to comprehensively develop and utilize Camellia oleifera resources, compounds in extracts from C. oleifera leaves were separated and identified. Three extracted parts of II, III and VIII from C. oleifera leaves were separated, purified and collected by using macroporous resin and column chromatography, and eight high-purity compounds were achieved by HPLC preparation. Four compounds were identified by methods of TLC colouring, high resolution LC-MS, and NMR, including dihydroresveratrol （1）, quercetin （3）, quercetin 3-O-β-D-rhamnoside （6） and 1-（3′, 5′-dimethoxy） phenyl-2-[4″-O-β-D-glucopyranosyl （6→1）-O-α-L-rhamnopyranosyl] phenylethane （8）. Among the four compounds, the compounds 3 and 6 were very popular flavonoids in C. oleifera, and this was the second time that the compounds 8 was reported, but the extraction rate was higher than that in the first report. This was the first time that dihydroresveratrol was reported in Camellia.

关 键 词：液相色谱 制备 高清晰质谱 结构鉴定 核磁 

分 类 号：S609[农业科学—园艺学] S794.4