杨木同步糖化发酵生产乳酸的条件优化  

The optimization of SSF conditions for poplar woods to lactic acid

作　　者：林丽云[1] 王义强[1,2,3] 马国辉[1] 吴腾飞[1] 

机构地区：[1]中南林业科技大学生物技术实验室,湖南长沙410004 [2]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004 [3]中南林业科技大学国家林业局生物乙醇研究中心,湖南长沙410004

出　　处：《中南林业科技大学学报》2015年第10期138-141,共4页Journal of Central South University of Forestry & Technology

基　　金：国家林业局"948"项目"木质纤维生物炼制丁醇汽油及聚乳酸生物塑料技术引进"(2011-4-13)

摘　　要：以经过蒸汽爆破预处理后的杨木为原料,利用戊糖乳杆菌突变株进行同步糖化发酵。用高效液相色谱法测定发酵液中的乳酸含量,对酶解温度、酶解p H、纤维素酶添加量进行单因素试验分析,对发酵温度、发酵p H、接种量进行单因素试验分析,再通过正交试验对发酵条件进行优化。研究发现,最佳发酵条件:发酵温度为40℃,发酵p H为5.7,接种量为6%,纤维素酶添加量为15 FPU/g,乳酸产量为9.42 g/L,产酸量提高了83.62%。利用价格低廉且来源广泛的杨木发酵生产乳酸,具有广阔的工业应用前景。In this paper, poplar woods,which was pretreated by steam explosion,was used for SSF with mutant lactobacillus pentosus. The content of lactic acid was measured by HPLC, analysis on the enzymatic temperature,the enzymatic pH, the amount of cellulase separately, after analysis on the fermentation temperature,the fermentation PH,the inoculation amount, the fermentation conditions was optimized by orthogonal experiments. The results showed that the optimum fermentation conditions was as follows： the fermentation temperature was 40 ℃, the fermentation pH was 5.7, the inoculation amount was 6%, the amount of cellulase was 15 FPU/g and the yield of lactic acid was 9.42 g/L which raised 83.62% compared with the original conditions. Taking advantage of the cheap and extensive poplar woods for the production of lactic acid will have a broad prospect for industrial application.

关 键 词：杨木 同步糖化发酵 乳酸 优化 

分 类 号：S792.11[农业科学—林木遗传育种]