肝缺血再灌注损伤对大鼠肺组织HO-1和iNOS表达的影响  

Expression of HO-1 and iNOS in lung tissues in rats after hepatic ischemia-reperfusion injury

作　　者：谭敬[1] 周荣胜[1] 刘庆波[1] 马正敏[1] 刘齐宁[1] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,西安710061

出　　处：《山西医科大学学报》2015年第9期861-865,共5页Journal of Shanxi Medical University

基　　金：陕西省科技计划基金资助项目(2006K14-G2-(9))

摘　　要：目的探讨肝缺血再灌注损伤对大鼠肺组织血红素氧合酶-1(HO-1)和诱导型一氧化氮合酶(i NOS)表达的影响。方法选取健康SD大鼠40只,随机分为假手术组(sham组)和肝缺血再灌注组(IR组),每组20只。建立70%肝缺血再灌注损伤大鼠模型。使用图像分析系统测定肺组织免疫组化切片中HO-1、i NOS蛋白平均吸光度值,镜下观察HE染色肝肺组织病理改变,测量肺叶湿/干重比(W/D),检测丙二醛(MDA)含量、髓过氧化物酶(MPO)及超氧化物歧化酶(SOD)的活性。结果 HE染色肝肺组织病理改变,IR组肝肺损伤较sham组重。与sham组比较,IR组肺组织W/D、MDA和MPO表达明显升高(P<0.05),肺组织SOD表达明显降低(P<0.05),肺组织HO-1和i NOS蛋白表达明显升高(P<0.05)。结论肝缺血再灌注损伤能加重大鼠肺损伤,上调肺组织中的HO-1及i NOS表达。Objective To investigate the effect of hepatic ischemia-reperfusion on the expression of HO-1 and iNOS in lung tissues of rats. Methods Forty healthy SD rats were randomly divided into two groups （ n = 20） ： sham group and hepatic ischemia reperfusion group（IR）. The rat model of hepatic ischemia and reperfusion with 70% of hepatic portal blood flow blocking was established. Patho- logical changes of liver and lung tissues were observed by HE staining. The average absorbance values of HO-1 and iNOS protein ex- pression in lung tissues were detected by immunohistochemistry. The lung wet/dry ratio, content of malondialdehyde （MDA） , activity of superoxide dismutase（SOD） and myeloperoxidase （MPO） were detected. Results HE staining of liver and lung tissues showed the liver and lung injury in IR group was more serious than in sham group. Lung wet/dry weight ratio in IR group was significantly higher than in sham group（P 〈 0.05 ）. Lung wet/dry weight ratio, content of MDA, activity of MPO in lung tissues in IR group were signifi- cantly higher than in sham group（ P 〈0. 05 ）. The activity of SOD in lung tissues in IR group was significantly lower than in sham group （P 〈 0. 05 ）. In lung tissue, the average absorbance values of HO-1 protein and iNOS protein in IR group were significantly higher than in sham group （P 〈 0.05）. Conclusion Hepatic ischemia-reperfusion can aggravate the lung injury in rats and its mechanism may be related to the increase of HO-1 and iNOS protein expression.

关 键 词：肝缺血再灌注损伤 肺 HO-1 INOS 

分 类 号：R363[医药卫生—病理学]