肠易激综合征患者炎症因子与claudin-1的相关性  

Correlation between inflammatory cytokines and claudin-1 in patients with irritable bowel syndrome

作　　者：黄小平[1] 刘欣[1] 王燕[1] 王学勤[1] 龚均[1] 

机构地区：[1]西安交通大学第二附属医院消化科,西安710004

出　　处：《山西医科大学学报》2015年第9期876-880,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81100258);博福-益普生腹泻基金资助项目(IDF-2012-02)

摘　　要：目的测定肠易激综合征(irritable bowel syndrome,IBS)患者血清及结肠黏膜中肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)和紧密连接蛋白-1(claudin-1)的表达水平及其相关性,进一步了解IBS的发病机制。方法对22例IBS腹泻型(D-IBS)、18例IBS便秘型患者(C-IBS)、20例健康对照者应用ELISA法检测血清中TNF-α、IL-10、claudin-1的含量,免疫组化法检测结肠黏膜TNF-α、IL-10、claudin-1的表达。结果 1血清中TNF-α水平及结肠黏膜表达率D-IBS组均高于正常对照组(P<0.05);C-IBS组与正常对照组相比差异无统计学意义(P>0.05)。2血清IL-10水平及结肠黏膜表达率D-IBS组均低于正常对照组(P<0.05);C-IBS组与正常对照组相比差异无统计学意义(P>0.05)。3血清claudin-1水平及结肠黏膜表达率D-IBS组均低于正常对照组(P<0.05);C-IBS组均高于正常对照组(P<0.05)。4D-IBS组血清TNF-α与claudin-1成负相关(r=-0.867,P<0.05),血清IL-10与claudin-1成正相关(r=0.868,P<0.05)。结论 IBS腹泻症状的产生可能与炎性因子失衡而导致claudin-1改变有关。Objective To explore the pathogenesis of irritable bowel syndrome（IBS） by detecting the levels of tumor necrosis factor-α （TNF-α）, interleukin-10（IL-10） and tight junction protein-1 （claudin-1） in serum and colonic mucosa of patients with IBS and analy- zing their correlation. Methods The serum levels of TNF-α, IL-10 and claudin-1 were detected in 22 cases of diarrhea-type IBS （D- IBS）, 18 cases of constipated-type IBS（C-IBS）, and 20 healthy controls by ELISA. Meanwhile, the expression of TNF-α, IL-IO and claudin-1 was detected in colonic mucosa by immunohistochemistry. Results ①The level of TNF-ct was significantly higher in the se- rum and colonic mucosa in D-IBS group than that in control group（P 〈 0.05 ）, but there was no significant difference in the serum and the colonic mucosa between C-IBS group and control group （ P 〉 0.05 ）. ②The level of IL-10 in D-IBS group was significantly lower than that in control group （ P 〈 0.05 ）, but there was no significant difference in the serum and the colonic mucosa between C-IBS group and control group（P 〉 0.05 ）. ③The level of claudin-1 in D-IBS group was significantly lower in the serum and the colonic mu- cosa than that in control group（ P 〈 0.05 ）, but it was significantly higher in C-IBS group than that in control group（ P 〈 0.05 ）. ④The TNF-α level was negatively correlated with claudin-1 level in D-IBS group（ r = - 0. 867 ,P 〈 0.05 ） ,while the IL-10 level was positively correlated with claudin-1 level（ r = 0. 868,P 〈 0.05 ）. Conclusion The changes of claudin-1 are caused by the imbalance of inflam- matory cytokines ,which may be related to the symptom of diarrhea.

关 键 词：肠易激综合征 肿瘤坏死因子-Α 白介素-10 紧密连接蛋白-1 

分 类 号：R574.4[医药卫生—消化系统]