喉癌相关基因和miRNA综合数据库的构建  

Establishment of a comprehensive database for laryngeal cancer related genes and the miRNAs

作　　者：李孟娇[1] 鄂琪敏 刘加林[2] 黄婷婷[1] 梁传余[2] 

机构地区：[1]四川大学华西医院/华西临床医学院医学信息学教研室,成都610041 [2]四川大学华西医院/华西临床医学院耳鼻咽喉头颈外科,成都610041

出　　处：《中华耳鼻咽喉头颈外科杂志》2015年第9期765-768,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：基金项目：国家自然科学基金面上项目（71273182）;四川省科技支撑计划（2011FZ0035）

摘　　要：目的 对与喉癌相关基因和miRNA数据进行系统的整理和分析,建立有别于目前结构复杂、庞大的生物信息数据库,为喉癌的相关研究、教学提供方便、快捷的喉癌基因、miRNA主题的综合数据库.方法 基于B/S架构,采用Apache软件作为Web服务器,以MySQL作为数据库设计的编码语言并以PHP作为网页设计的编程语言,设计基因表、蛋白质表、miRNA表和喉癌患者相关临床信息表等表单,建立喉癌基因数据库.结果 目前数据库共收录了207个喉癌相关基因、243个蛋白质和26个miRNA;本数据库可以通过互联网进行访问和操作,并实现了对资料的浏览、检索等功能,并对数据库进行维护和更新;数据库包含了喉癌相关的基因、蛋白质和miRNA及其突变、甲基化、多样化表达等信息,以及喉癌相关分子实证研究的参考文献.结论 该数据库资源整合、界面友好,提高了喉癌相关基因的使用效率和效益.Objective By collecting and analyzing the laryngeal cancer related genes and the miRNAs,to build a comprehensive laryngeal cancer-related gene database,which differs from the current biological information database with complex and clumsy structure and focuses on the theme of gene and miRNA,and it could make the research and teaching more convenient and efficient.Methods Based on the B/S architecture,using Apache as a Web server,MySQL as coding language of database design and PHP as coding language of web design,a comprehensive database for laryngeal cancer-related genes was established,providing with the gene tables,protein tables,miRNA tables and clinical information tables of the patients with laryngeal cancer.Results The established database containsed 207 laryngeal cancer related genes,243 proteins,26 miRNAs,and their particular information such as mutations,methylations,diversified expressions,and the empirical references of laryngeal cancer relevant molecules.The database could be accessed and operated via the Internet,by which browsing and retrieval of the information were performed.The database were maintained and updated regularly.Conclusion The database for laryngeal cancer related genes is resource-integrated and user-friendly,providing a genetic information query tool for the study of laryngeal cancer.

关 键 词：喉肿瘤 肿瘤蛋白质类 ATP结合匣式转运子 微RNAS 数据库 

分 类 号：R739.65[医药卫生—肿瘤]