Cytotoxicity of mitochondrial-targeting silica-coated manganese oxide nanoparticles  

作　　者：Jie Wei Chao Yu Li Wang Jun Wang Zhiguo Zhou Hong Yang Shiping Yang 

机构地区：[1]The Key Laboratory of Resource Chemistry of Ministry of Education [2]Shanghai Key Laboratory of Rare Earth Functional Materials [3]Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors [4]Shanghai Normal University

出　　处：《Science China Chemistry》2015年第10期1537-1543,共7页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21271130,21371122);the Program for Changjiang Scholars and Innovative Research Team in University(IRT1269);the Shanghai Science and Technology Development Fund(12ZR1421800,13520502800);the Shanghai Pujiang Program(13PJ1406600);the Shanghai Municipal Education Commission(13ZZ110);Shanghai Normal University(SK201339);the International Joint Laboratory on Resource Chemistry

摘　　要：The mitochondrion is a promising target for diagnosis and therapy. Mitochondrial-targeting silica-coated manganese oxide nanoparticles(Mn O@Si O2-PPh3+ NPs) were successfully synthesized to explore the mitochondrial cytotoxicity of nanoparticles. The mitochondrial targeting property was confirmed by a laser scanning confocal microscopy experiment. Even after incubation for only 4 h, the cytotoxicity of Mn O@Si O2-PPh3+ NPs against cancer cells was obvious; the ATP content was significantly decreased to 40%; and the mitochondrial membrane potential was depleted. All of these results indicated the collapse of mitochondrial function and the start of a cell apoptosis pathway. Our findings suggest that mitochondrial-mediated apoptosis could be strengthened by targeting to the subcellular compartment.The mitochondrion is a promising target for diagnosis and therapy. Mitochondrial-targeting silica-coated manganese oxide nanoparticles（Mn O@Si O2-PPh3^＋ NPs） were successfully synthesized to explore the mitochondrial cytotoxicity of nanoparticles. The mitochondrial targeting property was confirmed by a laser scanning confocal microscopy experiment. Even after incubation for only 4 h, the cytotoxicity of Mn O@Si O2-PPh3^＋ NPs against cancer cells was obvious; the ATP content was significantly decreased to 40%; and the mitochondrial membrane potential was depleted. All of these results indicated the collapse of mitochondrial function and the start of a cell apoptosis pathway. Our findings suggest that mitochondrial-mediated apoptosis could be strengthened by targeting to the subcellular compartment.

关 键 词：mitochondrial targeting CYTOTOXICITY APOPTOSIS ATP content mitochondrial membrane potential 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学]